Stepwise artificial evolution of an Sw-5b immune receptor extends its resistance spectrum against resistance-breaking isolates of Tomato spotted wilt virus.
Stepwise artificial evolution of an Sw-5b immune receptor extends its resistance spectrum against resistance-breaking isolates of Tomato spotted wilt virus.
复制标题
Sw-5b 免疫受体的逐步人工进化扩大了其对番茄斑萎病毒耐药性破坏分离株的耐药谱。
DOI：
10.1111/pbi.13641
复制
发表时间：
2021-11
影响因子：
13.8
通讯作者：
Tao X
中科院分区：
文献类型：
--
作者：
Huang H;Huang S;Li J;Wang H;Zhao Y;Feng M;Dai J;Wang T;Zhu M;Tao X
植物使用细胞内核苷酸结合富含亮氨酸重复序列的免疫受体（NLR）来识别病原体编码的效应物并启动免疫应答。番茄斑萎病毒（Tomato spotted wilt virus，TSWV）是世界上最具破坏性的植物病毒之一，已被发现感染超过1000种植物物种。Sw‐ 5 b是番茄抗病育种中最有效、应用最广泛的抗TSWV基因。然而，携带Sw-5 b的番茄品种的广泛应用导致了抗性破坏（RB）TSWV的出现。因此，迫切需要新的有效基因来防止进一步的RB TSWV爆发。在这项研究中，我们进行了人工进化，以选择Sw-5 b突变体，可以扩展对TSWV RB分离物的抗性谱。与常规NLR不同，Sw-5 b在两步识别过程中使用N-末端茄科特异性结构域（SD）和C-末端LRR结构域检测病毒诱导子NSm。我们尝试通过涉及Sw-5 b的SD或LRR的随机诱变来选择功能获得突变体失败;因此，我们采取了逐步策略，首先在LRR中的R927残基处引入NSmRB响应突变，然后进行涉及Sw-5 b SD结构域的随机诱变。使用这种策略，我们获得了Sw-5 bL 33 P/K319 E/R927 A和Sw-5 bL 33 P/K319 E/R927 Q突变体，它们对携带NSmC 118 Y或NSmT 120 N突变的TSWV RB和其他美洲型番茄斑萎病毒有效。因此，我们能够扩展Sw-5 b的抗性谱;选择的Sw-5 b突变体将提供新的基因资源来控制RB TSWV。
Plants use intracellular nucleotide‐binding leucine‐rich repeat immune receptors (NLRs) to recognize pathogen‐encoded effectors and initiate immune responses. Tomato spotted wilt virus (TSWV), which has been found to infect >1000 plant species, is among the most destructive plant viruses worldwide. The Sw‐5b is the most effective and widely used resistance gene in tomato breeding to control TSWV. However, broad application of tomato cultivars carrying Sw‐5b has resulted in an emergence of resistance‐breaking (RB) TSWV. Therefore, new effective genes are urgently needed to prevent further RB TSWV outbreaks. In this study, we conducted artificial evolution to select Sw‐5b mutants that could extend the resistance spectrum against TSWV RB isolates. Unlike regular NLRs, Sw‐5b detects viral elicitor NSm using both the N‐terminal Solanaceae‐specific domain (SD) and the C‐terminal LRR domain in a two‐step recognition process. Our attempts to select gain‐of‐function mutants by random mutagenesis involving either the SD or the LRR of Sw‐5b failed; therefore, we adopted a stepwise strategy, first introducing a NSmRB‐responsive mutation at the R927 residue in the LRR, followed by random mutagenesis involving the Sw‐5b SD domain. Using this strategy, we obtained Sw‐5bL33P/K319E/R927A and Sw‐5bL33P/K319E/R927Q mutants, which are effective against TSWV RB carrying the NSmC118Y or NSmT120N mutation, and against other American‐type tospoviruses. Thus, we were able to extend the resistance spectrum of Sw‐5b; the selected Sw‐5b mutants will provide new gene resources to control RB TSWV.
登录
查看更多内容
发育调控拟南芥对番茄斑萎病毒感染的易感性
影响因子：
4.9
作者：
Huang, Ying;Hong, Hao;Tao, Xiaorong
通讯作者：
Tao, Xiaorong
影响因子：
4.9
作者：
Hallwass, Mariana;De Oliveira, Athos Silva;Kormelink, Richard
通讯作者：
Kormelink, Richard
影响因子：
13.8
作者：
Aravind, L
通讯作者：
Aravind, L
影响因子：
1.9
作者：
BOITEUX, LS;GIORDANO, LD
通讯作者：
GIORDANO, LD
影响因子：
11.6
作者：
Ellis, JG;Lawrence, GJ;Dodds, PN
通讯作者：
Dodds, PN