Biomarkers to identify Mycobacterium tuberculosis infection among borderline QuantiFERON results.
Biomarkers to identify Mycobacterium tuberculosis infection among borderline QuantiFERON results.
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DOI：
10.1183/13993003.02665-2021
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发表时间：
2022-08
期刊：
影响因子：
--
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中科院分区：
文献类型：
--
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Screening for tuberculosis (TB) infection often includes QuantiFERON-TB Gold Plus (QFT) testing. Previous studies showed that two-thirds of patients with negative QFT results just below the cut-off, so-called borderline test results, nevertheless had other evidence of TB infection. This study aimed to identify a biomarker profile by which borderline QFT results due to TB infection can be distinguished from random test variation. QFT supernatants of patients with a borderline (≥0.15 and <0.35 IU·mL−1), low-negative (<0.15 IU·mL−1) or positive (≥0.35 IU·mL−1) QFT result were collected in three hospitals. Bead-based multiplex assays were used to analyse 48 different cytokines, chemokines and growth factors. A prediction model was derived using LASSO regression and applied further to discriminate QFT-positive Mycobacterium tuberculosis-infected patients from borderline QFT patients and QFT-negative patients QFT samples of 195 patients were collected and analysed. Global testing revealed that the levels of 10 kDa interferon (IFN)-γ-induced protein (IP-10/CXCL10), monokine induced by IFN-γ (MIG/CXCL9) and interleukin-1 receptor antagonist in the antigen-stimulated tubes were each significantly higher in patients with a positive QFT result compared with low-negative QFT individuals (p<0.001). A prediction model based on IP-10 and MIG proved highly accurate in discriminating patients with a positive QFT (TB infection) from uninfected individuals with a low-negative QFT (sensitivity 1.00 (95% CI 0.79–1.00) and specificity 0.95 (95% CI 0.74–1.00)). This same model predicted TB infection in 68% of 87 patients with a borderline QFT result. This study was able to classify borderline QFT results as likely infection-related or random. These findings support additional laboratory testing for either IP-10 or MIG following a borderline QFT result for individuals at increased risk of reactivation TB. Additional laboratory testing of IP-10/CXCL10 and MIG/CXCL9 in individuals at increased risk of reactivation TB with a borderline QuantiFERON-TB Gold Plus result just below the formal cut-off helps to identify those with M. tuberculosis infection https://bit.ly/3t8zR7r
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影响因子：
1.8
作者：
Ruhwald M;Bjerregaard-Andersen M;Rabna P;Eugen-Olsen J;Ravn P
通讯作者：
Ravn P
影响因子：
1.6
作者：
Li Y;He D;Che Y;Zhao X
通讯作者：
Zhao X
影响因子：
9.4
作者：
Rubbo, Pierre-Alain;Nagot, Nicolas;Tuaillon, Edouard
通讯作者：
Tuaillon, Edouard
DOI：
10.1183/20734735.0079-2021
发表时间：
2021-09
期刊：
Breathe (Sheffield, England)
影响因子：
--
作者：
Migliori GB;Ong CWM;Petrone L;D'Ambrosio L;Centis R;Goletti D
通讯作者：
Goletti D
DOI：
10.1164/rccm.201203-0430oc
发表时间：
2013-01-15
影响因子：
24.7
作者：
Metcalfe, John Z.;Cattamanchi, Adithya;Graviss, Edward A.
通讯作者：
Graviss, Edward A.