Genomic Epidemiology of Carbapenemase Producing Klebsiella pneumoniae Strains at a Northern Portuguese Hospital Enables the Detection of a Misidentified Klebsiella variicola KPC-3 Producing Strain.
Genomic Epidemiology of Carbapenemase Producing Klebsiella pneumoniae Strains at a Northern Portuguese Hospital Enables the Detection of a Misidentified Klebsiella variicola KPC-3 Producing Strain.
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DOI：
10.3390/microorganisms8121986
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发表时间：
2020-12-13
期刊：
影响因子：
4.5
通讯作者：
Duarte A
中科院分区：
文献类型：
--
作者：
Perdigão J;Caneiras C;Elias R;Modesto A;Spadar A;Phelan J;Campino S;Clark TG;Costa E;Saavedra MJ;Duarte A
采用全基因组测序和生物信息学分析对来自葡萄牙北方Vila真实的地区的31株多重耐药肺炎克雷伯菌临床分离株的进化流行病学、耐药基因组、代谢基因组和活动基因组进行了表征。基因组群体结构由两种主要序列类型（ST）主导：ST 147（n = 17; 54.8%）和ST 15（n = 6; 19.4%），其包括四个不同的基因组簇。检测到两种主要碳青霉烯酶编码基因（blaKPC-3和blaOXA-48）沿着其他超广谱β-内酰胺酶编码基因座（blaCTX-M-15、blaSHV-12、blaSHV-27和blaSHV-187）。此外，全基因组测序使一个变异克雷伯氏菌KPC-3生产者分离物的鉴定成为可能，以前被误认为是K。pneumoniae，除了blaKPC-3碳青霉烯酶基因外，还携带染色体广谱β-内酰胺酶blaLEN-2编码基因、oqxAB和fosA耐药基因座。blaKPC-3基因定位于Tn 4401 b转座子（K. variicola n = 1; K. pneumoniae n = 2）和Tn 4401 d同种型（K. pneumoniae n = 2）。pneumoniae n = 28）。总之，我们的工作描述了blaKPC-3产生K.以及在感染控制措施期间在感染患者的监测培养物中检测到该物种。它还强调了额外控制措施的重要性，以克服产碳青霉烯酶克隆的克隆传播。
The evolutionary epidemiology, resistome, virulome and mobilome of thirty-one multidrug resistant Klebsiella pneumoniae clinical isolates from the northern Vila Real region of Portugal were characterized using whole-genome sequencing and bioinformatic analysis. The genomic population structure was dominated by two main sequence types (STs): ST147 (n = 17; 54.8%) and ST15 (n = 6; 19.4%) comprising four distinct genomic clusters. Two main carbapenemase coding genes were detected (blaKPC-3 and blaOXA-48) along with additional extended-spectrum β-lactamase coding loci (blaCTX-M-15, blaSHV-12, blaSHV-27, and blaSHV-187). Moreover, whole genome sequencing enabled the identification of one Klebsiella variicola KPC-3 producer isolate previously misidentified as K. pneumoniae, which in addition to the blaKPC-3 carbapenemase gene, bore the chromosomal broad spectrum β-lactamase blaLEN-2 coding gene, oqxAB and fosA resistance loci. The blaKPC-3 genes were located in a Tn4401b transposon (K. variicola n = 1; K. pneumoniae n = 2) and Tn4401d isoform (K. pneumoniae n = 28). Overall, our work describes the first report of a blaKPC-3 producing K. variicola, as well as the detection of this species during infection control measures in surveillance cultures from infected patients. It also highlights the importance of additional control measures to overcome the clonal dissemination of carbapenemase producing clones.
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影响因子：
9.5
作者：
Katoh K;Rozewicki J;Yamada KD
通讯作者：
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影响因子：
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1.7
作者：
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