bHLH010/089 Transcription Factors Control Pollen Wall Development via Specific Transcriptional and Metabolic Networks in Arabidopsis thaliana.
bHLH010/089 Transcription Factors Control Pollen Wall Development via Specific Transcriptional and Metabolic Networks in Arabidopsis thaliana.
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bHLH010/089 拟南芥转录因子通过特定转录和代谢网络控制花粉壁发育
DOI：
10.3390/ijms231911683
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发表时间：
2022-10-02
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
花粉壁是一种特殊的胞外细胞壁，它保护雄配子体免受各种环境胁迫的影响，并促进授粉。在这里，我们报道了bHLH010和bHLH089一起是花粉壁发育所必需的，通过调节它们特定的下游转录和代谢网络。Bhlh010和Bhlh089花粉粒的外壁和内壁结构均有严重缺陷。进一步的非靶向代谢和转录分析表明，bhlh010 bhlh089突变体中与花粉壁形态发生相关的代谢产物(包括多糖、甘油衍生物和黄酮醇)的积累发生了显著变化，与花粉壁形态发生相关的代谢酶编码基因和转运蛋白编码基因的表达下调。在这些下游靶基因中，CSLB03是一个新的靶点，目前还没有生物学功能的报道。我们发现bHLH010与CSLB03启动子上的两个E-box序列相互作用，并直接激活CSLB03的表达。Cslb03突变等位基因表现出类似bhlh010 bhlh089的花粉发育缺陷，大部分花粉粒具有缺陷的花粉壁结构。
The pollen wall is a specialized extracellular cell wall that protects male gametophytes from various environmental stresses and facilitates pollination. Here, we reported that bHLH010 and bHLH089 together are required for the development of the pollen wall by regulating their specific downstream transcriptional and metabolic networks. Both the exine and intine structures of bhlh010 bhlh089 pollen grains were severely defective. Further untargeted metabolomic and transcriptomic analyses revealed that the accumulation of pollen wall morphogenesis-related metabolites, including polysaccharides, glyceryl derivatives, and flavonols, were significantly changed, and the expression of such metabolic enzyme-encoding genes and transporter-encoding genes related to pollen wall morphogenesis was downregulated in bhlh010 bhlh089 mutants. Among these downstream target genes, CSLB03 is a novel target with no biological function being reported yet. We found that bHLH010 interacted with the two E-box sequences at the promoter of CSLB03 and directly activated the expression of CSLB03. The cslb03 mutant alleles showed bhlh010 bhlh089–like pollen developmental defects, with most of the pollen grains exhibiting defective pollen wall structures.
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影响因子：
7.4
作者：
Dobritsa, Anna A.;Lei, Zhentian;Sumner, Lloyd W.
通讯作者：
Sumner, Lloyd W.
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影响因子：
5.6
作者：
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通讯作者：
Liang C
影响因子：
7.2
作者：
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通讯作者：
Ma, Hong
影响因子：
7.4
作者：
Dardelle, Flavien;Lehner, Arnaud;Mollet, Jean-Claude
通讯作者：
Mollet, Jean-Claude
影响因子：
7.4
作者：
Chary, S. Narasimha;Hicks, Glenn R.;Raikhel, Natasha V.
通讯作者：
Raikhel, Natasha V.