Transient Presence of Live Leptospira interrogans in Murine Testes.
Transient Presence of Live Leptospira interrogans in Murine Testes.
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DOI：
10.1128/spectrum.02775-21
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发表时间：
2022-06-29
影响因子：
3.7
通讯作者：
Gomes-Solecki, Maria
中科院分区：
文献类型：
--
作者：
Shetty, Advait;Kundu, Suman;Vernel-Pauillac, Frederique;Ratet, Gwendoline;Werts, Catherine;Gomes-Solecki, Maria
分析钩端螺旋体在啮齿动物性器官中的传播和定殖具有重要价值，因为它质疑哺乳动物间性病传播的可能性。我们研究的目的是使用我们之前开发的感染模型评估小鼠睾丸中问号钩端螺旋体的存在和生存能力。采用亚致死和致死剂量的发光乳杆菌。在C57 BL/6小鼠睾丸中发现钩端螺旋体的存在，最早在感染后30分钟，最多持续到感染后3-4天。这在C3 H/HeJ小鼠致死感染后通过哥本哈根血清型的qPCR证实。在该模型中，未观察到睾丸的组织病理学变化。我们进一步研究了持久性血清型哥本哈根在C3 H/HeJ睾丸致死和亚致死感染后，不同剂量的钩端螺旋体。从致死性感染小鼠的睾丸中未回收到活钩端螺旋体。然而，我们发现19/19（100%）亚致死性感染小鼠的睾丸中的活培养钩端螺旋体在急性期，但不是在感染后15天，这对应于肾定植的慢性期。这些数据表明，睾丸与活的和潜在的感染性钩端螺旋体的殖民是短暂的，并限于螺旋体血症感染阶段。有必要进一步研究以评估小鼠睾丸中钩端螺旋体的存在是否导致精液中的排泄以及对雌性小鼠的性病传播。重要性钩端螺旋体的性传播分析对于确定是否发生直接动物间传播非常重要，这可能影响预防和治疗钩端螺旋体病的措施。本研究的目的是确定是否活钩端螺旋体定殖小鼠睾丸。我们发现，小鼠睾丸与活钩端螺旋体的定植是短暂的，仅限于急性螺旋体血症感染阶段，睾丸的短暂定植不足以引起组织病理学变化。
Analysis of Leptospira dissemination and colonization of sex organs in rodents is of significant value as it queries the possibility of mammal-to-mammal venereal transmission. The aim of our study was to evaluate the presence and viability of Leptospira interrogans in testes of mice using models of infection that we previously developed. Using sublethal and lethal doses of bioluminescent strains of L. interrogans serovars Manilae and Copenhageni, we visualized the presence of leptospires in testes of C57BL/6 mice as early as 30 min and up to days 3–4 postinfection. This was confirmed by qPCR for the Copenhageni serovar after lethal infection of C3H/HeJ mice. In this model, no histopathological changes were noticed in testis. We further studied persistence of serovar Copenhageni in C3H/HeJ testes after lethal and sublethal infection, with different doses of leptospires. No viable leptospires were recovered from testes of lethally infected mice. However, we found live culturable Leptospira in testes of 19/19 (100%) sublethally infected mice at the acute phase but not at 15 days postinfection, which corresponds to the chronic phase of renal colonization. The data suggest that colonization of testes with live and potentially infectious leptospires is transient and limited to the spirochetemic phase of infection. Further studies are necessary to evaluate if presence of Leptospira in testes of mice leads to excretion in semen and to venereal transmission to female mice. IMPORTANCE Analysis of venereal transmission of Leptospira is important to determine if direct animal to animal transmission occurs, which could impact measures to prevent and treat leptospirosis. The goal of this study was to determine if live Leptospira colonize mouse testes. We found that colonization of mouse testes with live Leptospira was transient and limited to the acute spirochetemic phase of infection and that transient colonization of the testes was insufficient to cause histopathological changes.
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影响因子：
3
作者：
Director, A.;Penna, B.;Lilenbaum, W.
通讯作者：
Lilenbaum, W.
影响因子：
3.1
作者：
Gomes, Charles K.;Guedes, Mariana;Gomes-Solecki, Maria
通讯作者：
Gomes-Solecki, Maria
影响因子：
5.1
作者：
HARRISON, NA;FITZGERALD, WR
通讯作者：
FITZGERALD, WR
影响因子：
2.2
作者：
Maiolino, Sergio Ricardo;Cortez, Adriana;Ribeiro, Marcio Garcia
通讯作者：
Ribeiro, Marcio Garcia
影响因子：
3.1
作者：
Monahan, Avril M.;Callanan, John J.;Nally, Jarlath E.
通讯作者：
Nally, Jarlath E.