Redescription of Paradiplozoon hemiculteri (Monogenea, Diplozoidae) from the type host Hemiculter leucisculus, with neotype designation.

Redescription of Paradiplozoon hemiculteri (Monogenea, Diplozoidae) from the type host Hemiculter leucisculus, with neotype designation.
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从模式宿主 Hemiculter leucisculus 中重新描述 Paradiplozoon hemiculteri(Monogenea、Diplozoidae),并命名为新型

DOI:
10.1051/parasite/2018004
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发表时间:
2018
期刊:
Parasite (Paris, France)
影响因子:
--
通讯作者:
Gelnar M
Gelnar M
中科院分区:
其他
文献类型:
--
作者:
Jirsová D;Ding X;Civáňová K;Jirounková E;Ilgová J;Koubková B;Kašný M;Gelnar M

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半囊副二倍体虫Paradiplozoon hemiculteri(Ling,1973)是寄生于亚洲鱼类鳃部的二倍体虫科成员。本种不仅没有模式标本,原始的描述也很差,有些矛盾,而且没有足够的分子数据。更重要的是,现有的形态学和分子数据是不一致的,波动很大。在这里,我们提出了一个重新描述的P. hemiculteri的基础上收集的模式寄主,尖腹Hemiculter leucisculus(Basilewsky,1855),在新模式产地(韶关,广东省,中国南部)的新分离物的形态和分子数据; P. hemiculteri的新模式被指定从这个集合。测量了体部、颊吸盘、咽部、附着夹、镰刀和中央钩柄的长度和宽度,并准确记录了正中板的前部和后部以及前后连接骨片的形状。基于第二rDNA内转录间隔区(ITS 2)序列的系统发育分析表明,所有新样品聚在一起,与GenBank中的半囊扁藻(P. hemiculteri)序列有明显差异。我们的研究结果证实,P. hemiculteri是唯一的二倍体已被证明是在鳃的H。leucisculus迄今为止。
Paradiplozoon hemiculteri (Ling, 1973), a member of the Diplozoidae, parasitizes the gills of Asian fish. Not only is the type material unavailable for this species, the original description was poor and somewhat conflicting, and adequate molecular data were not available. What is more, the available morphological and molecular data are inconsistent and fluctuate significantly. Here, we present a redescription of P. hemiculteri based on morphological and molecular data from new isolates collected from the type host, the sharpbelly Hemiculter leucisculus (Basilewsky, 1855), captured at the neotype locality (Shaoguan, Guangdong Province, southern China); a neotype for P. hemiculteri was designated from this collection. The length and width of the body, buccal suckers, pharynx, attachment clamps, sickle and the central hook handle were all measured and the shape of the anterior and posterior part of the median plate and anterior and posterior joining sclerites accurately documented. Phylogenetic analyses based on the sequences of the second rDNA internal transcribed spacer (ITS2) indicated that all new samples clustered together and differed clearly from sequences attributed to P. hemiculteri, which are deposited in GenBank. Our results confirm that P. hemiculteri is the only diplozoid that has demonstrably been found on the gills of H. leucisculus to date.
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