High-throughput imaging of adult fluorescent zebrafish with an LED fluorescence macroscope.
High-throughput imaging of adult fluorescent zebrafish with an LED fluorescence macroscope.
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DOI：
10.1038/nprot.2010.170
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发表时间：
2011-02
期刊：
影响因子：
14.8
通讯作者：
Langenau, David M.
中科院分区：
文献类型：
--
作者：
Blackburn, Jessica S.;Liu, Sali;Raimondi, Aubrey R.;Ignatius, Myron S.;Salthouse, Christopher D.;Langenau, David M.
斑马鱼是研究发育、行为、疾病和癌症的有用脊椎动物模型。斑马鱼的一个主要优点是，大量的动物可以经济地用于实验；然而，对成年活斑马鱼进行高通量成像的方法尚未开发。在这里，我们描述了构建发光二极管（LED）荧光宏观的方案，并使用它同时成像多达30只转基因表达荧光蛋白的成年动物，用荧光标记的肿瘤细胞移植或用荧光弹性体标记。这些方案表明，LED荧光显微镜能够区分五种荧光蛋白，并能同时在多个荧光通道中对未麻醉的成年斑马鱼进行成像。显微镜可以在1天内建立并用于成像，而根据所选择的方法，创建荧光标记的成年斑马鱼需要1小时到几个月的时间。LED荧光显微镜为快速筛选成年荧光斑马鱼提供了一种低成本、高通量的方法，可用于转基因动物成像、肿瘤植入筛选和个体标记以进行长期分析。
Zebrafish are a useful vertebrate model for the study of development, behavior, disease and cancer. A major advantage of zebrafish is that large numbers of animals can be economically used for experimentation; however, high-throughput methods for imaging live adult zebrafish had not been developed. Here, we describe protocols for building a light-emitting diode (LED) fluorescence macroscope and for using it to simultaneously image up to 30 adult animals that transgenically express a fluorescent protein, are transplanted with fluorescently labeled tumor cells or are tagged with fluorescent elastomers. These protocols show that the LED fluorescence macroscope is capable of distinguishing five fluorescent proteins and can image unanesthetized swimming adult zebrafish in multiple fluorescent channels simultaneously. The macroscope can be built and used for imaging within 1 day, whereas creating fluorescently labeled adult zebrafish requires 1 hour to several months, depending on the method chosen. The LED fluorescence macroscope provides a low-cost, high-throughput method to rapidly screen adult fluorescent zebrafish and it will be useful for imaging transgenic animals, screening for tumor engraftment, and tagging individual fish for long-term analysis.
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影响因子：
3.8
作者：
Ma, Rui;Taruttis, Adrian;Razansky, Daniel
通讯作者：
Razansky, Daniel
影响因子：
14.8
作者：
Mizgirev, Igor;Revskoy, Sergei
通讯作者：
Revskoy, Sergei
DOI：
10.1073/pnas.191380698
发表时间：
2001-09-25
影响因子：
11.1
作者：
Darland, T;Dowling, JE
通讯作者：
Dowling, JE
影响因子：
2
作者：
Kabli, Samira;Alia, A.;De Groot, Huub J. M.
通讯作者：
De Groot, Huub J. M.
影响因子：
10.5
作者：
Langenau, David M.;Keefe, Matthew D.;Zon, Leonard I.
通讯作者：
Zon, Leonard I.