Laser speckle flowmetry method for measuring spatial and temporal hemodynamic alterations throughout large microvascular networks.
Laser speckle flowmetry method for measuring spatial and temporal hemodynamic alterations throughout large microvascular networks.
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DOI：
10.1111/j.1549-8719.2012.00197.x
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发表时间：
2012-10
期刊：
影响因子：
--
通讯作者：
Price RJ
中科院分区：
文献类型：
--
作者：
Meisner JK;Sumer S;Murrell KP;Higgins TJ;Price RJ
1)开发并验证激光散斑血流仪（LSF）作为大型网络中单个微血管血流动力学的定量工具。2)使用LSF来确定C57 BL/6和BALB/c小鼠背侧皮褶微循环（DSFWC）的结构差异是否会对闭塞产生不同的网络血流动力学反应。我们比较了LSF速度测量已知/测量速度在体外使用毛细管组织幻影和在体内使用小鼠DSFWC和提睾肌。采用LSF法测定了C57 BL/6和BALB/c小鼠移植DSFWC后血流动力学的变化。在体外，我们发现归一化散斑强度（NSI）与速度的线性关系（R2≥0.97）不随直径或红细胞压积而变化，并且可以移动以满足预期的操作范围。在体内，DSFWC和提睾肌制备（R2=0.92和0.95，分别）表现出类似的NSI和中心线速度之间的线性关系。分层的小动脉到预测的侧支通路显示显着差异C57 BL/6和BALB/c株之间的饲料小动脉闭塞。这些数据表明，LSF的适用性，以活体显微镜微循环制剂确定相对和绝对的血流动力学在网络范围内的规模，同时保持个别微血管的分辨率。
1) Develop and validate laser speckle flowmetry (LSF) as a quantitative tool for individual microvessel hemodynamics in large networks. 2) Use LSF to determine if structural differences in the dorsal skinfold microcirculation (DSFWC) of C57BL/6 and BALB/c mice impart differential network hemodynamic responses to occlusion. We compared LSF velocity measurements to known/measured velocities in vitro using capillary tube tissue phantoms and in vivo using mouse DSFWCs and cremaster muscles. Hemodynamic changes induced by feed arteriole occlusion were measured using LSF in DSFWCs implanted on C57BL/6 and BALB/c. In vitro, we found that the normalized speckle intensity (NSI) versus velocity linear relationship (R2≥0.97) did not vary with diameter or hematocrit and can be shifted to meet an expected operating range. In vivo, DSFWC and cremaster muscle preparations (R2=0.92 and 0.95, respectively) demonstrated similar linear relationships between NSI and centerline velocity. Stratification of arterioles into predicted collateral pathways revealed significant differences between C57BL/6 and BALB/c strains in response to feed arteriole occlusion. These data demonstrate the applicability of LSF to intravital microscopy microcirculation preparations for determining both relative and absolute hemodynamics on a network-wide scale while maintaining the resolution of individual microvessels.
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DOI：
10.1111/j.1549-8719.2010.00051.x
发表时间：
2010-11
期刊：
Microcirculation (New York, N.Y. : 1994)
影响因子：
--
作者：
Meisner JK;Price RJ
通讯作者：
Price RJ
DOI：
10.1364/josaa.25.002088
发表时间：
2008-08
期刊：
Journal of the Optical Society of America. A, Optics, image science, and vision
影响因子：
--
作者：
Duncan DD;Kirkpatrick SJ
通讯作者：
Kirkpatrick SJ
DOI：
10.1152/ajpheart.00772.2008
发表时间：
2009-03-01
影响因子：
4.8
作者：
Distasi, Matthew R.;Case, Jamie;Unthank, Joseph L.
通讯作者：
Unthank, Joseph L.
影响因子：
4.4
作者：
Nagahara, Miyuki;Tamaki, Yasuhiro;Araie, Makoto
通讯作者：
Araie, Makoto
影响因子：
3.1
作者：
BAKER, M;WAYLAND, H
通讯作者：
WAYLAND, H