Spatial-Temporal Pattern of Sulfate-Dependent Anaerobic Methane Oxidation in an Intertidal Zone of the East China Sea
Spatial-Temporal Pattern of Sulfate-Dependent Anaerobic Methane Oxidation in an Intertidal Zone of the East China Sea
复制标题
东海潮间带硫酸盐依赖的厌氧甲烷氧化时空格局
DOI：
10.1128/aem.02638-18
复制
发表时间：
2019-02
影响因子：
4.4
通讯作者：
Hu Baolan
中科院分区：
文献类型：
--
作者：
Wang Jiaqi;Hua Miaolian;Cai Chaoyang;Hu Jiajie;Wang Junren;Yang Hongrui;Ma Fang;Qian Haifeng;Zheng Ping;Hu Baolan
由厌氧甲烷氧化菌（ANME）和硫酸盐还原菌（SRB）催化的硫酸盐依赖型厌氧甲烷氧化（S-AOM）过程是连接全球碳硫循环的重要环节。研究了S-AOM过程的时空分布特征和近岸潮间带沉积物中ANME古菌的分布特征。研究结果表明，S-AOM过程是潮间带生态系统中不可忽视的甲烷汇。我们还发现，ANME古菌对氧气敏感，喜欢厌氧环境条件。该研究将有助于我们更好地了解全球碳硫循环和温室气体减排，并为ANME古菌的富集提供新的视角。摘要甲烷是导致全球变暖的主要温室气体。由厌氧甲烷氧化菌（ANME）和硫酸盐还原菌（SRB）催化的硫酸盐依赖型厌氧甲烷氧化（S-AOM）过程是连接全球碳硫循环的重要环节，被认为是海洋生态系统中最重要的甲烷汇。然而，关于S-AOM过程的作用以及ANME古菌在潮间带生态系统中的分布研究却很少。潮间带是海陆之间的缓冲带，在全球地球化学循环中起着重要作用。本研究采用定量PCR、稳定同位素示踪和高通量测序技术，对潮间带ANME古菌的丰度、潜在甲烷氧化速率和群落结构进行了研究。结果表明，潜在的S-AOM活性范围为0至0.77 nmol 13CO2 g − 1（干沉积物）天− 1。ANME古菌的16S rRNA基因拷贝数达到106 × 107拷贝g-1（干沉积物）。S-AOM对总厌氧甲烷氧化的平均贡献率高达34.5%，而分解性厌氧甲烷氧化则占其余部分，表明S-AOM过程是潮间带生态系统不可忽视的甲烷汇。模拟柱实验还表明，ANME古菌对氧敏感，喜欢厌氧环境条件。该研究将有助于我们更好地了解全球碳硫循环和温室气体减排，并为ANME古菌的富集提供新的视角。由厌氧甲烷氧化菌（ANME）和硫酸盐还原菌（SRB）催化的硫酸盐依赖型厌氧甲烷氧化（S-AOM）过程是连接全球碳和硫循环的重要环节。研究了S-AOM过程的时空分布特征和近岸潮间带沉积物中ANME古菌的分布特征。研究结果表明，S-AOM过程是潮间带生态系统中不可忽视的甲烷汇。我们还发现，ANME古菌对氧气敏感，喜欢厌氧环境条件。该研究将有助于我们更好地了解全球碳硫循环和温室气体减排，并为ANME古菌的富集提供新的视角。
The sulfate-dependent anaerobic methane oxidation (S-AOM) process catalyzed by anaerobic methanotrophic (ANME) archaea and sulfate-reducing bacteria (SRB) is a vital link connecting the global carbon and sulfur cycles. We conducted a research into the spatial-temporal pattern of S-AOM process and the distribution of ANME archaea in coastal sediments collected from the intertidal zone. The results implied that S-AOM process was a methane sink that cannot be overlooked in the intertidal ecosystem. We also found that ANME archaea were sensitive to oxygen and preferred anaerobic environmental conditions. This study will help us gain a better understanding of the global carbon-sulfur cycle and greenhouse gas emission reduction and introduce a new perspective into the enrichment of ANME archaea. ABSTRACT Methane is a primary greenhouse gas which is responsible for global warming. The sulfate-dependent anaerobic methane oxidation (S-AOM) process catalyzed by anaerobic methanotrophic (ANME) archaea and sulfate-reducing bacteria (SRB) is a vital link connecting the global carbon and sulfur cycles, and it is considered to be the overriding methane sink in marine ecosystem. However, there have been few studies regarding the role of S-AOM process and the distribution of ANME archaea in intertidal ecosystem. The intertidal zone is a buffer zone between sea and land and plays an important role in global geochemical cycle. In the present study, the abundance, potential methane oxidation rate, and community structure of ANME archaea in the intertidal zone were studied by quantitative PCR, stable isotope tracing method and high-throughput sequencing. The results showed that the potential S-AOM activity ranged from 0 to 0.77 nmol 13CO2 g−1 (dry sediment) day−1. The copy number of 16S rRNA gene of ANME archaea reached 106 ∼ 107 copies g−1 (dry sediment). The average contribution of S-AOM to total anaerobic methane oxidation was up to 34.5%, while denitrifying anaerobic methane oxidation accounted for the rest, which implied that S-AOM process was an essential methane sink that cannot be overlooked in intertidal ecosystem. The simulated column experiments also indicated that ANME archaea were sensitive to oxygen and preferred anaerobic environmental conditions. This study will help us gain a better understanding of the global carbon-sulfur cycle and greenhouse gas emission reduction and introduce a new perspective into the enrichment of ANME archaea. IMPORTANCE The sulfate-dependent anaerobic methane oxidation (S-AOM) process catalyzed by anaerobic methanotrophic (ANME) archaea and sulfate-reducing bacteria (SRB) is a vital link connecting the global carbon and sulfur cycles. We conducted a research into the spatial-temporal pattern of S-AOM process and the distribution of ANME archaea in coastal sediments collected from the intertidal zone. The results implied that S-AOM process was a methane sink that cannot be overlooked in the intertidal ecosystem. We also found that ANME archaea were sensitive to oxygen and preferred anaerobic environmental conditions. This study will help us gain a better understanding of the global carbon-sulfur cycle and greenhouse gas emission reduction and introduce a new perspective into the enrichment of ANME archaea.
登录
查看更多内容
DOI：
--
发表时间：
2002
期刊：
--
影响因子：
--
作者：
T. Braak;F. Cateten;P. Šmilauer
通讯作者：
T. Braak;F. Cateten;P. Šmilauer
影响因子：
4.4
作者：
T. Treude;V. Orphan;K. Knittel;A. Gieseke;C. House;A. Boetius
通讯作者：
T. Treude;V. Orphan;K. Knittel;A. Gieseke;C. House;A. Boetius
影响因子：
3.7
作者：
C. Schubert;E. Durisch-Kaiser;Christian P. Holzner;Lucia Klauser;B. Wehrli;O. Schmale;J. Greinert;D. McGinnis;M. De Batist;R. Kipfer
通讯作者：
C. Schubert;E. Durisch-Kaiser;Christian P. Holzner;Lucia Klauser;B. Wehrli;O. Schmale;J. Greinert;D. McGinnis;M. De Batist;R. Kipfer
影响因子：
4.4
作者：
F. V. D. Nat;J. D. Brouwer;J. Middelburg;H. J. Laanbroek
通讯作者：
F. V. D. Nat;J. D. Brouwer;J. Middelburg;H. J. Laanbroek
影响因子：
4.2
作者：
Nunoura, Takuro;Oida, Hanako;Horikoshi, Koki
通讯作者：
Horikoshi, Koki