Streptozotocin-Induced Hyperglycemia Is Associated with Unique Microbiome Metabolomic Signatures in Response to Ciprofloxacin Treatment.
Streptozotocin-Induced Hyperglycemia Is Associated with Unique Microbiome Metabolomic Signatures in Response to Ciprofloxacin Treatment.
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DOI：
10.3390/antibiotics11050585
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发表时间：
2022-04-27
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影响因子：
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众所周知，微生物组在人类健康中起着关键作用，例如抗生素对该系统的损害具有有害影响。因此，人们普遍认识到，微生物组中的脱靶抗生素敏感性是一个特别麻烦的副作用，对宿主的健康有严重影响。因此，一个紧迫的研究领域是微生物组内抗生素敏感性决定因素的表征，因为了解这些机制可能为微生物组保护治疗策略的发展提供信息。特别是，已知代谢环境在这些微生物群落对抗生素的不同反应中起关键作用。在这里，我们探讨宿主血糖异常对小鼠盲肠环丙沙星敏感性的作用。我们使用16S rRNA测序和非靶向代谢组学的组合来表征微生物组分类和环境的变化。我们发现血糖异常对环丙沙星相关的微生物组结构变化影响最小。然而，从代谢的角度来看，宿主高血糖与呼吸、中枢碳代谢和核苷酸合成相关代谢物的显著变化有关。综上所述，这些数据表明宿主血糖可能会影响抗生素挑战期间微生物组的功能。
It is well recognized that the microbiome plays key roles in human health, and that damage to this system by, for example, antibiotic administration has detrimental effects. With this, there is collective recognition that off-target antibiotic susceptibility within the microbiome is a particularly troublesome side effect that has serious impacts on host well-being. Thus, a pressing area of research is the characterization of antibiotic susceptibility determinants within the microbiome, as understanding these mechanisms may inform the development of microbiome-protective therapeutic strategies. In particular, metabolic environment is known to play a key role in the different responses of this microbial community to antibiotics. Here, we explore the role of host dysglycemia on ciprofloxacin susceptibility in the murine cecum. We used a combination of 16S rRNA sequencing and untargeted metabolomics to characterize changes in both microbiome taxonomy and environment. We found that dysglycemia minimally impacted ciprofloxacin-associated changes in microbiome structure. However, from a metabolic perspective, host hyperglycemia was associated with significant changes in respiration, central carbon metabolism, and nucleotide synthesis-related metabolites. Together, these data suggest that host glycemia may influence microbiome function during antibiotic challenge.
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DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
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EPA-NG：遗传序列的大量平行进化位置。
影响因子：
6.5
作者：
Barbera P;Kozlov AM;Czech L;Morel B;Darriba D;Flouri T;Stamatakis A
通讯作者：
Stamatakis A
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
7.4
作者：
Boettcher, Christoph;Chapman, Alexandra;Glawischnig, Erich
通讯作者：
Glawischnig, Erich
影响因子：
7.4
作者：
Fuhrer, Tobias;Heer, Dominik;Zamboni, Nicola
通讯作者：
Zamboni, Nicola