Complementary genetic and genomic approaches help characterize the linkage group I seed protein QTL in soybean.
Complementary genetic and genomic approaches help characterize the linkage group I seed protein QTL in soybean.
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DOI：
10.1186/1471-2229-10-41
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发表时间：
2010-03-03
影响因子：
5.3
通讯作者：
Vance CP
中科院分区：
文献类型：
--
作者：
Bolon YT;Joseph B;Cannon SB;Graham MA;Diers BW;Farmer AD;May GD;Muehlbauer GJ;Specht JE;Tu ZJ;Weeks N;Xu WW;Shoemaker RC;Vance CP
许多作物的营养和经济价值实际上是种子蛋白质和油含量的函数。深入了解这些成分在发育种子中沉积的遗传和分子控制机制，以指导作物改良。在大豆（Glycine max（L.）梅里尔）对种子蛋白质含量有显著影响。大豆近等基因系（NIL）对在种子蛋白质方面存在差异并且在含有LG I蛋白质QTL的渗入基因组区段方面不同，被用作资源来划分QTL区域并研究发育种子中转录本丰度的变化。LG I QTL区域被描绘为20号染色体上小于8.4Mbp的基因组序列。使用Affyphyrin ® Soy GeneChip和高通量Illumina®全转录组测序平台，鉴定了在NIL之间显示显著种子转录物积累差异的13个基因，其定位到8.4Mbp LG I蛋白质QTL区域。本研究鉴定了LG I蛋白质QTL上的候选基因，这些基因可能参与大豆种子中蛋白质含量的调节。结果表明，互补的方法来表征对比近等离子体，并提供全基因组转录组的洞察力，了解种子生物学和大豆基因组的力量。
The nutritional and economic value of many crops is effectively a function of seed protein and oil content. Insight into the genetic and molecular control mechanisms involved in the deposition of these constituents in the developing seed is needed to guide crop improvement. A quantitative trait locus (QTL) on Linkage Group I (LG I) of soybean (Glycine max (L.) Merrill) has a striking effect on seed protein content. A soybean near-isogenic line (NIL) pair contrasting in seed protein and differing in an introgressed genomic segment containing the LG I protein QTL was used as a resource to demarcate the QTL region and to study variation in transcript abundance in developing seed. The LG I QTL region was delineated to less than 8.4 Mbp of genomic sequence on chromosome 20. Using Affymetrix® Soy GeneChip and high-throughput Illumina® whole transcriptome sequencing platforms, 13 genes displaying significant seed transcript accumulation differences between NILs were identified that mapped to the 8.4 Mbp LG I protein QTL region. This study identifies gene candidates at the LG I protein QTL for potential involvement in the regulation of protein content in the soybean seed. The results demonstrate the power of complementary approaches to characterize contrasting NILs and provide genome-wide transcriptome insight towards understanding seed biology and the soybean genome.
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影响因子：
7.2
作者：
Borisjuk, L;Rolletschek, H;Weber, H
通讯作者：
Weber, H
影响因子：
7
作者：
Ben Amor, Besma;Wirth, Sonia;Crespi, Martin
通讯作者：
Crespi, Martin
影响因子：
7.4
作者：
Dam, Svend;Laursen, Brian S.;Stougaard, Jens
通讯作者：
Stougaard, Jens
影响因子：
2.3
作者：
BRIM, CA;BURTON, JW
通讯作者：
BURTON, JW
影响因子：
7.2
作者：
Borisjuk, L;Walenta, S;Wobus, U
通讯作者：
Wobus, U