Transformation, Proliferation, and Influence on Host Performance by Three Gut Bacteria in the Rice Water Weevil (Coleoptera: Curculionidae)
Transformation, Proliferation, and Influence on Host Performance by Three Gut Bacteria in the Rice Water Weevil (Coleoptera: Curculionidae)
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稻水象甲三种肠道细菌的转化、增殖及其对寄主性能的影响（鞘翅目：象甲科）
DOI：
10.1093/aesa/saw097
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发表时间：
2017-01
影响因子：
2.3
通讯作者：
Jiang Mingxing
中科院分区：
文献类型：
--
作者：
Huang Yunshan;Huang Xu;Jiang Mingxing
摘要稻水象甲Lissorhoptrus oryzophilus Kuschel是美国和东亚地区水稻的主要害虫之一。几个共生细菌已被分离出肠道的象鼻虫，但没有一个已经被评估的潜在的副transgenesis生物防治的候选人。我们用DsRed标记的质粒转化了三种细菌菌株，粪肠球菌、蜡状芽孢杆菌和从象鼻虫中分离的肠杆菌。然后我们研究了它们在宿主肠道中的命运。为了评估它们对宿主的生物学影响，我们测量了成虫的消费，存活，产卵量和孵化率后，象鼻虫接种转化细菌。我们的结果表明，三种细菌中的每一种都可以成功转化。接种后2d肠道细菌密度较低。然而，它们的种群数量在第二天急剧增加，无论是喂食还是饥饿，并在接种后4天达到高峰。每种菌株在成虫肠道中定殖至少9-15 d，主要在后肠中积累。转化菌株对象鼻虫的生产性能没有明显的影响。总之，由于能够定殖宿主肠道和易于培养，这些细菌可能是有前途的副transgenesis候选人。它们可以被工程化并重新引入成年稻水象甲的肠道中，可能产生能够损害象甲发育和生存的效应分子。
Abstract The rice water weevil, Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae), is one of the most serious pests of rice in the United States and East Asia. Several symbiotic bacteria have been isolated from the gut of this weevil, but none of them have been evaluated for the potential to be paratransgenesis candidates for biological control. We transformed three bacterial strains, Enterococcus faecalis, Bacillus cereus, and Enterobacter sp. isolated from the weevil, with a DsRed-labeled plasmid. We then investigated their fate in the host gut. To assess their biological impacts on the host, we measured adult consumption, survival, egg production, and hatch rate after weevils were inoculated with the transformed bacteria. Our results showed that each of the three bacteria could be successfully transformed. The intestinal bacterial densities were low 2 d after inoculation. However, their populations increased dramatically the following day, regardless whether the adults were fed or starved, and reached their peak abundance 4 d after inoculation. Each strain colonized the adult gut for at least 9–15 d, accumulating mostly in the hindgut. No apparent effects by the transformed strains were observed on weevil performance. In conclusion, due to the ability to colonize the host gut and to ease of culturing, these bacteria might be promising paratransgenesis candidates. They can be engineered and re-introduced into the gut of adult rice water weevils potentially producing effector molecules capable of impairing weevil development and survival.
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影响因子：
4.4
作者：
Adams, Aaron S.;Aylward, Frank O.;Raffa, Kenneth F.
通讯作者：
Raffa, Kenneth F.
影响因子：
4
作者：
K. Watanabe;K. Abe;M. Sato
通讯作者：
K. Watanabe;K. Abe;M. Sato
影响因子：
2.6
作者：
J. Peloquin;L. Kuzina;C. Lauzon;T. Miller
通讯作者：
J. Peloquin;L. Kuzina;C. Lauzon;T. Miller
影响因子：
4
作者：
Crippen, T. L.;Zheng, L.;Yu, Z.
通讯作者：
Yu, Z.
DOI：
10.1007/978-94-009-0775-1_15
发表时间：
1990
期刊：
--
影响因子：
--
作者：
M. Way
通讯作者：
M. Way