Blue-light-independent activity of Arabidopsis cryptochromes in the regulation of steady-state levels of protein and mRNA expression.
Blue-light-independent activity of Arabidopsis cryptochromes in the regulation of steady-state levels of protein and mRNA expression.
复制标题
拟南芥隐花色素在调节蛋白质和 mRNA 表达稳态水平方面的不依赖蓝光的活性。
DOI：
10.1093/mp/ssm018
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发表时间：
2008
期刊：
影响因子：
27.5
通讯作者：
Lin,Chen-Tao
中科院分区：
文献类型：
--
作者：
Yang,Yue-Jun;Zuo,Ze-Cheng;Zhao,Xiao-Ying;Li,Xu;Klejnot,John;Li,Yan;Chen,Ping;Liang,Song-Ping;Yu,Xu-Hong;Liu,Xuan-Ming;Lin,Chen-Tao
隐色素是蓝光受体，介导蓝光对拟南芥下胚轴伸长的抑制和蓝光对花形成的刺激。除了蓝光依赖性功能外，隐色素还参与了生物钟、子叶展开和下胚轴抑制的蓝光非依赖性调节。然而，与隐色素不依赖蓝光功能相关的分子机制尚不清楚。我们在这里报道了一项比较蛋白质组学研究，研究了光对蛋白质表达的调节。我们发现，正如预期的那样，许多代谢酶的蛋白质表达在蓝光和红光下都发生了变化。令人惊讶的是，在蓝光和红光下，cry1cry2突变体中的一些光调节蛋白表达变化受到损害。这一结果表明，隐色素除了介导蛋白质表达的蓝光依赖性调控外，还参与了基因表达的蓝光非依赖性调控。与这一假设一致，cry1cry2突变体不仅在蓝光下，而且在红光下表现出mRNA表达变化的减少，尽管隐色素对红光依赖性基因表达变化的影响通常不太明显。这些结果支持了一种假设，即隐色素除了具有蓝光特异性功能外，还在红光/远红光受体光敏色素介导的基因表达控制中发挥作用。
Cryptochromes are blue-light receptors that mediate blue-light inhibition of hypocotyl elongation and blue-light stimulation of floral initiation inArabidopsis. In addition to their blue-light-dependent functions, cryptochromes are also involved in blue-light-independent regulation of the circadian clock, cotyledon unfolding, and hypocotyl inhibition. However, the molecular mechanism associated with the blue-light-independent function of cryptochromes remains unclear. We reported here a comparative proteomics study of the light regulation of protein expression. We showed that, as expected, the protein expression of many metabolic enzymes changed in response to both blue light and red light. Surprisingly, some light-regulated protein expression changes are impaired in thecry1cry2mutant in both blue light and red light. This result suggests that, in addition to mediating blue-light-dependent regulation of protein expression, cryptochromes are also involved in the blue-light-independent regulation of gene expression. Consistent with this hypothesis, thecry1cry2mutant exhibited reduced changes of mRNA expression in response to not only blue light, but also red light, although the cryptochrome effects on the red-light-dependent gene expression changes are generally less pronounced. These results support a hypothesis that, in addition to their blue-light-specific functions, cryptochromes also play roles in the control of gene expression mediated by the red/far-red-light receptor phytochromes.
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佛得角群岛隐花色素 2 等位基因在没有蓝光的情况下增强拟南芥子叶的展开1[w]
影响因子：
7.4
作者：
J. Botto;C. Alonso‐Blanco;Ignacio Garzarón;R. Sánchez;J. Casal
通讯作者：
J. Casal
DOI：
10.1073/pnas.95.5.2686
发表时间：
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影响因子：
11.1
作者：
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作者：
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影响因子：
56.9
作者：
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影响因子：
56.9
作者：
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通讯作者：
Deng, XW