Ecological diversification reveals routes of pathogen emergence in endemic Vibrio vulnificus populations
Ecological diversification reveals routes of pathogen emergence in endemic Vibrio vulnificus populations
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生态多样化揭示了地方性创伤弧菌种群中病原体出现的途径
DOI：
10.1073/pnas.2103470118
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发表时间：
2021
影响因子：
11.1
通讯作者：
Almagro-Moreno, S
中科院分区：
文献类型：
--
作者：
López-Pérez, M;Jayakumar, JM;Grant, TA;Zaragoza-Solas, A;Cabello-Yeves, PJ;Almagro-Moreno, S
病原体的出现是一种复杂的现象，尽管与公共卫生相关，但人们对其仍知之甚少。创伤弧菌是一种新出现的人类病原体，可引起致命的败血症，死亡率超过 50%。迄今为止，导致临床菌株出现的生态驱动因素以及允许这些克隆在人类宿主上定植的独特遗传特征仍然大多未知。我们最近调查了佛罗里达州东部的一个大河口，那里经常发生这种疾病的爆发，并发现了这种细菌的地方性种群。我们建立了两个采样点，观察到位置与致病潜力之间存在很强的相关性。一个位点显着富集了属于一个系统基因组簇 (C1) 的菌株，大多数临床菌株都属于该簇。有趣的是，从该位点分离的菌株表现出与临床结果相关的表型特征，而来自第二位点的菌株属于很少引起人类疾病的簇（C2）。对 C1 基因组的分析表明，临床相关等位基因形式的独特遗传标记在毒力中具有潜在作用。最后，对采样点的宏基因组和理化分析表明，这种显着的簇分布和遗传特征与不同的生物和非生物因素（例如盐度、营养物质或生物多样性）密切相关，揭示了生态系统如何产生选择压力，促进群体中具有致病潜力的特定菌株的出现。这些知识可用于评估环境来源中病原体出现的风险，并可用于制定预防未来疫情爆发的新策略。
Pathogen emergence is a complex phenomenon that, despite its public health relevance, remains poorly understood.Vibrio vulnificus, an emergent human pathogen, can cause a deadly septicaemia with over 50% mortality rate. To date, the ecological drivers that lead to the emergence of clinical strains and the unique genetic traits that allow these clones to colonize the human host remain mostly unknown. We recently surveyed a large estuary in eastern Florida, where outbreaks of the disease frequently occur, and found endemic populations of the bacterium. We established two sampling sites and observed strong correlations between location and pathogenic potential. One site is significantly enriched with strains that belong to one phylogenomic cluster (C1) in which the majority of clinical strains belong. Interestingly, strains isolated from this site exhibit phenotypic traits associated with clinical outcomes, whereas strains from the second site belong to a cluster that rarely causes disease in humans (C2). Analyses of C1 genomes indicate unique genetic markers in the form of clinical-associated alleles with a potential role in virulence. Finally, metagenomic and physicochemical analyses of the sampling sites indicate that this marked cluster distribution and genetic traits are strongly associated with distinct biotic and abiotic factors (e.g., salinity, nutrients, or biodiversity), revealing how ecosystems generate selective pressures that facilitate the emergence of specific strains with pathogenic potential in a population. This knowledge can be applied to assess the risk of pathogen emergence from environmental sources and integrated toward the development of novel strategies for the prevention of future outbreaks.
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DOI：
--
发表时间：
1995
期刊：
Proceedings. International Conference on Intelligent Systems for Molecular Biology
影响因子：
--
作者：
S. Eddy
通讯作者：
S. Eddy
创伤弧菌的血清存活：基因型、荚膜、补体、临床起源和原位培养的作用
影响因子：
3.7
作者：
T. Williams;M. Ayrapetyan;H. Ryan;J. Oliver
通讯作者：
J. Oliver
影响因子：
4.3
作者：
H. Sambrook
通讯作者：
H. Sambrook
影响因子：
3.6
作者：
Baker-Austin, Craig;McArthur, J. V.;Stepanauskas, Ramunas
通讯作者：
Stepanauskas, Ramunas
影响因子：
6.4
作者：
Lopez-Perez, Mario;Jayakumar, Jane M.;Almagro-Moreno, Salvador
通讯作者：
Almagro-Moreno, Salvador