Kynurenine Metabolism in the Fat Body Non-autonomously Regulates Imaginal Disc Repair in Drosophila.
Kynurenine Metabolism in the Fat Body Non-autonomously Regulates Imaginal Disc Repair in Drosophila.
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DOI：
10.1016/j.isci.2020.101738
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发表时间：
2020-12-18
期刊：
影响因子：
5.8
通讯作者：
Miura M
中科院分区：
文献类型：
--
作者：
Kashio S;Miura M
组织的相互作用是维持体内平衡的关键，然而，很少有人知道远程组织如何调节再生。在此之前，我们建立了一个遗传双系统，诱导细胞消融果蝇幼虫成虫盘，同时操纵基因在非受损组织。使用体液代谢组分析和遗传损伤系统，我们发现脂肪体中的色氨酸（Trp）-犬尿氨酸（Kyn）途径是椎间盘修复所需的。对脂肪体中Trp-Kyn代谢的遗传操纵会损害椎间盘再生，但不会影响翅膀发育。特别是，脂肪体来源的体液犬尿烯酸（KynA）是椎间盘修复所必需的。在椎间盘修复的早期阶段，脂肪体中甲硫氨酸（Met）合成S-腺苷甲硫氨酸（SAM）的损伤阻碍了血淋巴中KynA水平的维持，这表明Met-SAM和Trp-Kyn代谢之间存在联系。我们的数据表明，来自脂肪体的KynA作为组织修复和再生的容许代谢物。果蝇幼虫脂肪体中的Trp-Kyn通路是椎间盘修复的远程需要脂肪体来源的体液KynA是椎间盘修复所需的脂肪体中的SAM合成影响椎间盘修复期间血淋巴中的KynA水平生物科学;细胞生物学;发育生物学;分子生物学
Tissue interactions are critical for maintaining homeostasis; however, little is known about how remote tissue regulates regeneration. Previously, we established a genetic dual system that induces cell ablation in Drosophila larval imaginal discs and simultaneously manipulates genes in non-damaged tissues. Using humoral metabolome analysis and a genetic damage system, we found that the Tryptophan (Trp)-Kynurenine (Kyn) pathway in the fat body is required for disc repair. Genetic manipulation of Trp-Kyn metabolism in the fat body impaired disc regeneration without affecting wing development. In particular, the fat body-derived humoral kynurenic acid (KynA) was required for disc repair. The impairment of S-adenosylmethionine (SAM) synthesis from methionine (Met) in the fat body hampers the maintenance of KynA levels in hemolymph at the early stage of disc repair, suggesting a connection between Met-SAM and Trp-Kyn metabolisms. Our data indicate KynA from the fat body acts as a permissive metabolite for tissue repair and regeneration. Trp-Kyn pathway in Drosophila larval fat body is remotely required for disc repair The fat body-derived humoral KynA is required for disc repair SAM synthesis in the fat body affects KynA levels in hemolymph during disc repair Biological Sciences; Cell Biology; Developmental Biology; Molecular Biology
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DOI：
10.1016/j.cub.2010.01.038
发表时间：
2010-03-09
期刊：
Current biology : CB
影响因子：
--
作者：
Halme A;Cheng M;Hariharan IK
通讯作者：
Hariharan IK
影响因子：
64.5
作者：
Fontana L;Partridge L
通讯作者：
Partridge L
影响因子：
4.6
作者：
Fox, Donald T.;Cohen, Erez;Smith-Bolton, Rachel
通讯作者：
Smith-Bolton, Rachel
影响因子：
64.8
作者：
Mazur, Pawel K.;Reynoird, Nicolas;Khatri, Purvesh;Jansen, Pascal W. T. C.;Wilkinson, Alex W.;Liu, Shichong;Barbash, Olena;Van Aller, Glenn S.;Huddleston, Michael;Dhanak, Dashyant;Tummino, Peter J.;Kruger, Ryan G.;Garcia, Benjamin A.;Butte, Atul J.;Vermeulen, Michiel;Sage, Julien;Gozani, Or
通讯作者：
Gozani, Or
影响因子：
46.9
作者：
Kennerdell, JR;Carthew, RW
通讯作者：
Carthew, RW