Nanoscale Visualization of Bacterial Microcompartments Using Atomic Force Microscopy.
Nanoscale Visualization of Bacterial Microcompartments Using Atomic Force Microscopy.
复制标题
使用原子力显微镜对细菌微区室进行纳米级可视化。
作者：
Jorge Rodriguez;M. Faulkner;Lu
细菌微区室（BMC）是原核生物中存在的一种多面体蛋白质细胞器，在代谢增强中起着重要作用。由于它们的自组装和模块化性质，近年来，BMCs获得了越来越多的兴趣，其目的是构建新的纳米生物反应器和支架，以促进细胞代谢和分子递送。在本章中，我们描述了原子力显微镜（AFM）技术作为一种方法来研究自组装动力学和BMC的物理性质。我们专注于样品的制备，测量过程，和数据分析的高速AFM成像和纳米压痕为基础的光谱，这是用来确定组装动力学的BMC壳蛋白和完整的BMC结构的纳米力学，分别。所描述的方法可以应用于其他类型的自组装生物细胞器的研究。
Bacterial microcompartments (BMCs) are polyhedral protein organelles in many prokaryotes, playing significant roles in metabolic enhancement. Due to their self-assembly and modularity nature, BMCs have gained increased interest in recent years, with the intent of constructing new nanobioreactors and scaffolding to promote cellular metabolisms and molecule delivery. In this chapter, we describe the technique of atomic force microscopy (AFM) as a method to study the self-assembly dynamics and physical properties of BMCs. We focus on the sample preparation, the measurement procedure, and the data analysis for high-speed AFM imaging and nanoindentation-based spectroscopy, which were used to determine the assembly dynamics of BMC shell proteins and the nanomechanics of intact BMC structures, respectively. The described methods could be applied to the study of other types of self-assembling biological organelles.
细菌微型室内细胞器：蛋白质壳的结构和进化。
影响因子：
12.4
作者：
Yeates TO;Crowley CS;Tanaka S
通讯作者：
Tanaka S
DOI：
10.1016/j.jmbbm.2017.05.039
发表时间：
2017-10
影响因子：
3.9
作者：
Sicard D;Fredenburgh LE;Tschumperlin DJ
通讯作者：
Tschumperlin DJ