Crystal structures of RNase H2 in complex with nucleic acid reveal the mechanism of RNA-DNA junction recognition and cleavage.
Crystal structures of RNase H2 in complex with nucleic acid reveal the mechanism of RNA-DNA junction recognition and cleavage.
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DOI：
10.1016/j.molcel.2010.11.001
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发表时间：
2010-11-24
期刊：
影响因子：
16
通讯作者：
Nowotny M
中科院分区：
文献类型：
--
作者：
Rychlik MP;Chon H;Cerritelli SM;Klimek P;Crouch RJ;Nowotny M
两类RNase H水解RNA/DNA杂合体的RNA。与需要四个核糖核苷酸切割的RNA酶H1相反，RNA酶H2可以切割含有单个核糖核苷酸的双链DNA，这表明不同的体内底物。本文报道了2型核糖核酸酶H与含有（5′）RNA-DNA（3′）连接的底物复合物的晶体结构。他们揭示了一种独特的识别和底物辅助切割机制。一个保守的酪氨酸残基扭曲的核酸在交界处，使基板的催化功能，通过参与协调的活性位点的金属离子。RNase H2的生物化学和结构特性解释了该酶对连接底物的偏好，并建立了与RNase H1的结构和机制差异。连接识别对于去除嵌入DNA中的RNA是重要的，并且可能在DNA复制和修复中起重要作用。<$RNase H2与核酸底物复合物的结构研究<$RNA-DNA（3′）连接的特异性识别机制揭示了连接的特异性变形导致底物辅助催化
Two classes of RNase H hydrolyze RNA of RNA/DNA hybrids. In contrast to RNase H1 that requires four ribonucleotides for cleavage, RNase H2 can nick duplex DNAs containing a single ribonucleotide, suggesting different in vivo substrates. We report here the crystal structures of a type 2 RNase H in complex with substrates containing a (5′)RNA-DNA(3′) junction. They revealed a unique mechanism of recognition and substrate-assisted cleavage. A conserved tyrosine residue distorts the nucleic acid at the junction, allowing the substrate to function in catalysis by participating in coordination of the active site metal ion. The biochemical and structural properties of RNase H2 explain the preference of the enzyme for junction substrates and establish the structural and mechanistic differences with RNase H1. Junction recognition is important for the removal of RNA embedded in DNA and may play an important role in DNA replication and repair. ► Structures of RNase H2 in complex with nucleic acid substrate reported ► The mechanism of specific recognition of (5′)RNA-DNA(3′) junction revealed ► Specific deformation of the junction leads to substrate-assisted catalysis
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影响因子：
14.9
作者：
Lavery R;Moakher M;Maddocks JH;Petkeviciute D;Zakrzewska K
通讯作者：
Zakrzewska K
DOI：
10.1111/j.1742-4658.2009.06909.x
发表时间：
2009-03
期刊：
The FEBS journal
影响因子：
--
作者：
Champoux JJ;Schultz SJ
通讯作者：
Schultz SJ
影响因子：
16
作者：
Nowotny, Marcin;Gaidamakov, Sergei A.;Yang, Wei
通讯作者：
Yang, Wei
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
影响因子：
5.6
作者：
Chapados, BR;Chai, Q;Tainer, JA
通讯作者：
Tainer, JA