Genome-wide analysis of the potato Hsp20 gene family: identification, genomic organization and expression profiles in response to heat stress.
Genome-wide analysis of the potato Hsp20 gene family: identification, genomic organization and expression profiles in response to heat stress.
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马铃薯 Hsp20 基因家族的全基因组分析：鉴定、基因组组织和响应热应激的表达谱
DOI：
10.1186/s12864-018-4443-1
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发表时间：
2018-01-18
期刊：
影响因子：
4.4
通讯作者：
Chen Q
中科院分区：
文献类型：
--
作者：
Zhao P;Wang D;Wang R;Kong N;Zhang C;Yang C;Wu W;Ma H;Chen Q
热休克蛋白(HSPs)是植物在各种非生物胁迫下耐受机制中的重要组成部分。热休克蛋白HSP20是主要的热休克蛋白家族，而马铃薯(Solanum Tuberosum)是一种重要的热敏性蔬菜作物，对HSP20家族的研究很少。为了揭示马铃薯Hsp20s应对非生物胁迫的机制，利用生物信息学方法对马铃薯Hsp20基因家族进行了分析。总共鉴定了48个马铃薯Hsp20基因(StHsp20s)，并根据它们的染色体位置进行了命名。序列分析表明，大多数StHsp20基因(89.6%)不含内含子或只有一个内含子。系统发育分析表明，除10个基因外，所有的StHsp20基因都属于12个亚家族。48个StHsp20基因随机分布在12条染色体上。共鉴定出19个串联重复的StHsp20s和1对节段性重复基因(StHsp20-15和StHsp20-48)。顺式元件分析推测，除StHsp20-41外，StHsp20s至少含有一个胁迫响应顺式元件。StHsp20基因家族的热图显示，除StHsp20-2和StHsp20-45外，其他基因在各种组织和器官中都有表达。用实时荧光定量聚合酶链式反应检测了StHsp20基因的表达水平，结果表明，StHsp20基因对多种非生物胁迫，如热、盐或干旱胁迫都有响应。在高温胁迫下，14个StHsp20基因(StHsp20-4、6、7、9、20、21、33、34、35、37、41、43、44和46)的相对表达水平显著上调(100倍以上)。这些结果为阐明StHsp20家族的进化关系和进一步研究StHsp20基因的功能特性提供了有价值的信息。
Heat shock proteins (Hsps) are essential components in plant tolerance mechanism under various abiotic stresses. Hsp20 is the major family of heat shock proteins, but little of Hsp20 family is known in potato (Solanum tuberosum), which is an important vegetable crop that is thermosensitive. To reveal the mechanisms of potato Hsp20s coping with abiotic stresses, analyses of the potato Hsp20 gene family were conducted using bioinformatics-based methods. In total, 48 putative potato Hsp20 genes (StHsp20s) were identified and named according to their chromosomal locations. A sequence analysis revealed that most StHsp20 genes (89.6%) possessed no, or only one, intron. A phylogenetic analysis indicated that all of the StHsp20 genes, except 10, were grouped into 12 subfamilies. The 48 StHsp20 genes were randomly distributed on 12 chromosomes. Nineteen tandem duplicated StHsp20s and one pair of segmental duplicated genes (StHsp20-15 and StHsp20-48) were identified. A cis-element analysis inferred that StHsp20s, except for StHsp20-41, possessed at least one stress response cis-element. A heatmap of the StHsp20 gene family showed that the genes, except for StHsp20-2 and StHsp20-45, were expressed in various tissues and organs. Real-time quantitative PCR was used to detect the expression level of StHsp20 genes and demonstrated that the genes responded to multiple abiotic stresses, such as heat, salt or drought stress. The relative expression levels of 14 StHsp20 genes (StHsp20-4, 6, 7, 9, 20, 21, 33, 34, 35, 37, 41, 43, 44 and 46) were significantly up-regulated (more than 100-fold) under heat stress. These results provide valuable information for clarifying the evolutionary relationship of the StHsp20 family and in aiding functional characterization of StHsp20 genes in further research.
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