An RNA-Seq-Based Framework for Characterizing Canine Prostate Cancer and Prioritizing Clinically Relevant Biomarker Candidate Genes.
An RNA-Seq-Based Framework for Characterizing Canine Prostate Cancer and Prioritizing Clinically Relevant Biomarker Candidate Genes.
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DOI：
10.3390/ijms222111481
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发表时间：
2021-10-25
影响因子：
5.6
通讯作者：
Murua Escobar H
中科院分区：
文献类型：
--
作者：
Thiemeyer H;Taher L;Schille JT;Packeiser EM;Harder LK;Hewicker-Trautwein M;Brenig B;Schütz E;Beck J;Nolte I;Murua Escobar H
犬前列腺癌（PCa）是一种高度恶性的疾病，类似于其人类对应物。与人类的情况相反，促进犬前列腺癌风险分层的多基因方法几乎没有建立。本研究的目的是表征犬PCa的转录景观，并通过多步筛选方法鉴定诊断、预后和/或治疗生物标志物。对10例恶性肿瘤组织和14例非恶性肿瘤组织和细针穿刺活检标本进行RNA测序，以寻找差异表达基因（DEG）和失调通路。共检测到4098个DEG，涉及49条通路。这49条通路可分为5条超级通路，总结犬PCa的特征：（i）炎症反应和细胞因子;（ii）免疫系统和细胞死亡的调节;（iii）细胞表面和PI 3 K信号传导;（iv）细胞周期;以及（v）吞噬体和自噬。在作为一个或多个超级通路成员的高度去调控、中度至强烈表达的DEG中，169个DEG在相关数据库和/或文献中列出，包括PCa通路、致癌基因、前列腺特异性基因和可药用基因的成员。这些基因是新的和有前途的候选诊断，预后和/或治疗犬PCa生物标志物。
Prostate cancer (PCa) in dogs is a highly malignant disease akin to its human counterpart. In contrast to the situation in humans, multi-gene approaches facilitating risk stratification of canine PCa are barely established. The aims of this study were the characterization of the transcriptional landscape of canine PCa and the identification of diagnostic, prognostic and/or therapeutic biomarkers through a multi-step screening approach. RNA-Sequencing of ten malignant tissues and fine-needle aspirations (FNA), and 14 nonmalignant tissues and FNAs was performed to find differentially expressed genes (DEGs) and deregulated pathways. The 4098 observed DEGs were involved in 49 pathways. These 49 pathways could be grouped into five superpathways summarizing the hallmarks of canine PCa: (i) inflammatory response and cytokines; (ii) regulation of the immune system and cell death; (iii) cell surface and PI3K signaling; (iv) cell cycle; and (v) phagosome and autophagy. Among the highly deregulated, moderately to strongly expressed DEGs that were members of one or more superpathways, 169 DEGs were listed in relevant databases and/or the literature and included members of the PCa pathway, oncogenes, prostate-specific genes, and druggable genes. These genes are novel and promising candidate diagnostic, prognostic and/or therapeutic canine PCa biomarkers.
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影响因子：
50.3
作者：
Hanahan, Douglas;Coussens, Lisa M.
通讯作者：
Coussens, Lisa M.
影响因子：
3.1
作者：
CHAPDELAINE, P;GAUTHIER, E;DUBE, JY
通讯作者：
DUBE, JY
影响因子：
2.4
作者：
Fonseca-Alves, Carlos Eduardo;Kobayashi, Priscila Emiko;Laufer-Amorim, Renee
通讯作者：
Laufer-Amorim, Renee
影响因子：
51.1
作者：
Cuzick, Jack;Swanson, Gregory P.;Fisher, Gabrielle;Brothman, Arthur R.;Berney, Daniel M.;Reid, Julia E.;Mesher, David;Speights, V. O.;Stankiewicz, Elzbieta;Foster, Christopher S.;Moller, Henrik;Scardino, Peter;Warren, Jorja D.;Park, Jimmy;Younus, Adib;Flake, Dart D., II;Wagner, Susanne;Gutin, Alexander;Lanchbury, Jerry S.;Stone, Steven
通讯作者：
Stone, Steven
影响因子：
2.6
作者：
Fonseca-Alves CE;Kobayashi PE;Palmieri C;Laufer-Amorim R
通讯作者：
Laufer-Amorim R