Host susceptibility factors render ripe tomato fruit vulnerable to fungal disease despite active immune responses.
Host susceptibility factors render ripe tomato fruit vulnerable to fungal disease despite active immune responses.
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尽管有主动免疫反应，但宿主的敏感性因素使成熟的番茄果实容易受到真菌疾病的影响。
DOI：
10.1093/jxb/eraa601
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发表时间：
2021-03-29
影响因子：
6.9
通讯作者：
Blanco-Ulate B
中科院分区：
文献类型：
--
作者：
Silva CJ;van den Abeele C;Ortega-Salazar I;Papin V;Adaskaveg JA;Wang D;Casteel CL;Seymour GB;Blanco-Ulate B
成熟水果对真菌病原体的敏感性增加，对作物生产和适销性构成重大威胁。在这里，我们将转录组分析与突变体研究结合起来，以揭示与番茄（Solanum lycopersicum）果实的防御和易感性相关的关键过程。使用接种三种真菌病原体的未成熟和成熟水果，我们确定了依赖于几丁质酶、WRKY转录因子和活性氧解毒的常见病原体反应。我们确定，防御反应的强度和多样性不会显着影响相互作用的结果，因为易受影响的成熟水果对病原体感染产生了强烈的免疫反应。然后，为了区分可能导致易感性的成熟特征，我们利用了对真菌感染表现出不同易感性模式的未成熟番茄突变体。根据转录和激素分析，易受影响的番茄基因型在维持细胞氧化还原稳态方面存在损失，而茉莉酸的积累和信号传导与抗性果实中的防御激活相一致。我们鉴定并验证了一个易感因子：果胶酸裂解酶 (PL)。基于 CRISPR 的 PL 敲除（而非多聚半乳糖醛酸酶 (PG2a)）可将成熟果实的敏感性降低 50% 以上。这项研究表明，针对促进易感性的特定基因是提高番茄果实对真菌病害的抵抗力的可行策略。番茄成熟过程中对真菌病害的易感性增加是由易感因子（特别是果胶降解酶）的积累驱动的。
The increased susceptibility of ripe fruit to fungal pathogens poses a substantial threat to crop production and marketability. Here, we coupled transcriptomic analyses with mutant studies to uncover critical processes associated with defense and susceptibility in tomato (Solanum lycopersicum) fruit. Using unripe and ripe fruit inoculated with three fungal pathogens, we identified common pathogen responses reliant on chitinases, WRKY transcription factors, and reactive oxygen species detoxification. We established that the magnitude and diversity of defense responses do not significantly impact the interaction outcome, as susceptible ripe fruit mounted a strong immune response to pathogen infection. Then, to distinguish features of ripening that may be responsible for susceptibility, we utilized non-ripening tomato mutants that displayed different susceptibility patterns to fungal infection. Based on transcriptional and hormone profiling, susceptible tomato genotypes had losses in the maintenance of cellular redox homeostasis, while jasmonic acid accumulation and signaling coincided with defense activation in resistant fruit. We identified and validated a susceptibility factor, pectate lyase (PL). CRISPR-based knockouts of PL, but not polygalacturonase (PG2a), reduced susceptibility of ripe fruit by >50%. This study suggests that targeting specific genes that promote susceptibility is a viable strategy to improve the resistance of tomato fruit against fungal disease. Increased susceptibility to fungal disease during tomato ripening is driven by the accumulation of susceptibility factors, particularly a pectin degrading enzyme.
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影响因子：
4.2
作者：
Ahmadi-Afzadi, Masoud;Orsel, Mathilde;Renou, Jean-Pierre
通讯作者：
Renou, Jean-Pierre
影响因子：
5.3
作者：
Andolfo G;Jupe F;Witek K;Etherington GJ;Ercolano MR;Jones JD
通讯作者：
Jones JD
影响因子：
7.4
作者：
Fantini, Elio;Falcone, Giulia;Giuliano, Giovanni
通讯作者：
Giuliano, Giovanni
影响因子：
2.7
作者：
Chiu, C. -M.;You, B. -J.;Lee, M. -H.
通讯作者：
Lee, M. -H.
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y