Bactericidal Effect of Photolysis of H2O2 in Combination with Sonolysis of Water via Hydroxyl Radical Generation.
Bactericidal Effect of Photolysis of H2O2 in Combination with Sonolysis of Water via Hydroxyl Radical Generation.
复制标题
DOI：
10.1371/journal.pone.0132445
复制
发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Niwano Y
中科院分区：
文献类型：
--
作者：
Sheng H;Nakamura K;Kanno T;Sasaki K;Niwano Y
研究了过氧化氢(H_2O_2)光解与超声波联合作用产生的羟基自由基(·OH)在H_2O_2水溶液同时受到激光和超声波照射时产生的·OH产率增加的杀菌效果。用激光(波长：405 nm，辐照度：46和91 mW/cm2)和超声波(功率：30w，频率：1.65 MHz)在水温20±1℃下同时照射金黄色葡萄球菌悬液2min。与单独激光照射过氧化氢相比，激光和超声波联合照射显著减少了活细菌的数量。相比之下，单独的超声波照射几乎没有杀菌作用。这些结果表明，H_2O_2的光解和水的超声分解对杀菌活性的联合作用是协同的。多因素方差分析还表明，过氧化氢浓度、激光功率和超声辐射的交互作用对杀菌活性有显著影响。DNA氧化损伤评价结果表明，与单独激光照射相比，激光和超声波联合作用对细菌DNA具有明显的氧化损伤作用，因此认为激光和超声波联合作用对细菌DNA的杀菌活性可能是通过对DNA等细胞成分的氧化损伤来实现的。
The bactericidal effect of hydroxyl radical (·OH) generated by combination of photolysis of hydrogen peroxide (H2O2) and sonolysis of water was examined under the condition in which the yield of ·OH increased additively when H2O2 aqueous solution was concomitantly irradiated with laser and ultrasound. The suspension of Staphylococcus aureus mixed with the different concentrations of H2O2 was irradiated simultaneously with a laser light (wavelength: 405 nm, irradiance: 46 and 91 mW/cm2) and ultrasound (power: 30 w, frequency: 1.65 MHz) at 20 ± 1°C of the water bulk temperature for 2 min. The combination of laser and ultrasound irradiation significantly reduced the viable bacterial count in comparison with the laser irradiation of H2O2 alone. By contrast, the ultrasound irradiation alone exerted almost no bactericidal effect. These results suggested that the combination effect of photolysis of H2O2 and sonolysis of water on bactericidal activity was synergistic. A multi-way analysis of variance also revealed that the interaction of H2O2 concentration, laser power and ultrasound irradiation significantly affected the bactericidal activity. Since the result of oxidative DNA damage evaluation demonstrated that the combination of laser and ultrasound irradiation significantly induced oxidative damage of bacterial DNA in comparison with the laser irradiation of H2O2 alone, it was suggested that the combination effect of photolysis of H2O2 and sonolysis of water on bactericidal activity would be exerted via oxidative damage of cellular components such as DNA.
登录
查看更多内容
影响因子：
64.5
作者：
Kohanski, Michael A.;Dwyer, Daniel J.;Collins, James J.
通讯作者：
Collins, James J.
影响因子：
2.4
作者：
Iwasawa, Atsuo;Saito, Keita;Niwano, Yoshimi
通讯作者：
Niwano, Yoshimi
影响因子：
4.9
作者：
PITT, WG;MCBRIDE, MO;SAGERS, RD
通讯作者：
SAGERS, RD
影响因子：
4
作者：
Nakamura, Keisuke;Kanno, Taro;Kohno, Masahiro
通讯作者：
Kohno, Masahiro
DOI：
10.1143/jjap.40.3493
发表时间：
2001-05-01
期刊：
JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS SHORT NOTES & REVIEW PAPERS
影响因子：
--
作者：
Fukuda, S;Ogawa, R;Kondo, T
通讯作者：
Kondo, T