Role of Sperm Sulfogalactosylglycerolipid in Mouse Sperm-Zona Pellucida Binding1
Role of Sperm Sulfogalactosylglycerolipid in Mouse Sperm-Zona Pellucida Binding1
复制标题
精子磺基半乳糖甘油脂在小鼠精子透明带结合中的作用1
DOI：
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发表时间：
2000
影响因子：
3.6
通讯作者：
N. Tanphaichitr
中科院分区：
文献类型：
--
作者：
D. White;W. Weerachatyanukul;B. Gadella;Nuanthip Kamolvarin;M. Attar;N. Tanphaichitr
摘要磺基半乳糖甘油脂（SGG）是哺乳动物雄性生殖细胞的主要硫基糖脂。与其他磺基糖脂一样，SGG被认为参与细胞-细胞/细胞外基质粘附。具体而言，我们研究了精子SGG是否在精卵相互作用中发挥作用。最初，我们生产了亲和纯化的兔多克隆免疫球蛋白（IG）G抗体，其特异性识别SGG（抗SGG）。用抗SGG IgG间接免疫荧光法将SGG定位于小鼠精子头部的凸脊、凹脊和顶体后。用抗SGG IgG/Fab预处理精子以浓度依赖性方式抑制精子-透明带（ZP）体外结合（最高达62%）。在初级结合水平观察到这种抑制。用抗SGG IgG处理的精子发生自发和ZP诱导的顶体反应的速率与用免疫前兔血清IgG处理的对照精子相同。荧光标记的SGG脂质体被证明与卵ZP特异性相关，而荧光标记的半乳糖基甘油脂质（SGG的亲本脂质）和磷脂酰丝氨酸（如SGG带负电荷）的脂质体则没有。此外，SGG脂质体与精子和分离的ZP共孵育以浓度依赖性方式抑制精子与ZP的结合。这些结果强烈表明精子SGG参与了与ZP的直接结合。
Abstract Sulfogalactosylglycerolipid (SGG) is the major sulfoglycolipid of mammalian male germ cells. Like other sulfoglycolipids, SGG is believed to be involved in cell-cell/extracellular matrix adhesion. Specifically, we investigated whether sperm SGG played a role in sperm-egg interaction. Initially, we produced an affinity-purified, rabbit polyclonal immunoglobulin (Ig) G antibody that specifically recognized SGG (anti-SGG). Indirect immunofluorescence using anti-SGG IgG localized SGG to the convex and concave ridges and the postacrosome of the mouse sperm head. Pretreatment of sperm with anti-SGG IgG/Fab inhibited sperm-zona pellucida (ZP) binding in vitro in a concentration-dependent manner (to a maximum of 62%). This inhibition was observed at the level of primary binding. Sperm treated with anti-SGG IgG underwent the spontaneous and ZP-induced acrosome reaction at the same rate as control sperm treated with preimmune rabbit serum IgG. Fluorescently labeled SGG liposomes were shown to associate specifically with the egg ZP, whereas fluorescently labeled liposomes of galactosylglycerolipid (SGG's parental lipid) and phosphatidylserine (negatively charged like SGG) did not. Furthermore, coincubation of SGG liposomes with sperm and isolated ZP inhibited sperm-ZP binding in a concentration-dependent manner. These results strongly suggest an involvement of sperm SGG in direct binding to the ZP.
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影响因子：
56.9
作者：
HAROUSE, JM;BHAT, S;GONZALEZSCARANO, F
通讯作者：
GONZALEZSCARANO, F
兔精子膜自身抗原 RSA 的表征，作为凝集素样透明带结合蛋白。
影响因子：
2.7
作者：
O'Rand,MG;Widgren,EE;Fisher,SJ
通讯作者：
Fisher,SJ
通过糖脂-玻璃缀合物体外免疫制备磺酰氧基半乳糖甘油脂单克隆抗体。
影响因子：
2.2
作者：
Eddy,EM;Muller,CH;Lingwood,CA
通讯作者：
Lingwood,CA
DOI：
10.1139/o85-134
发表时间：
1985
期刊：
Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire
影响因子：
--
作者：
Lingwood,CA
通讯作者：
Lingwood,CA
DOI：
10.1016/0005-2736(91)90295-j
发表时间：
1991-01-30
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
MACDONALD, RC;MACDONALD, RI;HU, LR
通讯作者：
HU, LR