SNP-based linkage mapping for validation of adult plant stripe rust resistance QTL in common wheat cultivar Chakwal 86
SNP-based linkage mapping for validation of adult plant stripe rust resistance QTL in common wheat cultivar Chakwal 86
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基于SNP的连锁作图验证普通小麦品种Chakwal 86成株条锈病抗性QTL
DOI：
10.1016/j.cj.2018.12.002
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发表时间：
2019-04
期刊：
影响因子：
6.6
通讯作者：
Kang Zhensheng
中科院分区：
文献类型：
--
作者：
Zeng Qingdong;Wu Jianhui;Huang Shuo;Yuan Fengping;Liu Shengjie;Wang Qilin;Mu Jingmei;Yu Shizhou;Chen Li;Han Dejun;Kang Zhensheng
中国的小麦作物不断受到条锈病的挑战。推广具有不同抗性的品种是防治该病的最佳策略。利用抗病品种Chakwal 86与感病地方品种明县169杂交，在多种环境下对重组自交系(RIL)群体进行了抗条锈病遗传研究和数量性状基因定位。利用小麦35K SNP芯片对128个重组自交系进行了基因分型，构建了包含1480个多态SNP标记的全基因组连锁图谱。位于1BL和3BS染色体上的两个主效QTL和位于6BS染色体上的一个微效QTL对降低条锈病严重程度有显著效果。利用复合区间作图(CIM)和包含复合区间作图(ICIM)对QTL进行验证。这些方法解释了59.0%-74.1%的疾病反应表型变异。1BL上的QTL为Yr29/Lr46，3BS上的QTL为CIMMYT种质中的抗性等位基因，而不是Yr30/Sr2。6BS上的QTL可能与已知的QTL相对应。含有联合QTL的重组自交系比含有单个QTL或无QTL的重组自交系抗性更强。对3BS染色体上效应最大的QTL进行了Kasp分析，对部分重组自交系和150个小麦无关系进行了分析。3BS上的QTL及其连锁的KASP标记可用于分子标记辅助选择，以提高育种工作中的抗条锈性。
Wheat crops in China are constantly challenged by stripe rust. Deployment of cultivars with diverse resistances is the best strategy to control the disease. A recombinant inbred line (RIL) population derived from a cross between the resistant cultivar Chakwal 86 and susceptible landrace Mingxian 169 was studied in multiple environments to examine the underlying genetics and to identify quantitative trait loci (QTL) for stripe rust resistance. One hundred and twenty-eight RILs were genotyped with wheat 35K SNP array and a genome-wide linkage map with 1480 polymorphic SNP markers, or bins, was constructed. Two major QTL on chromosomes 1BL and 3BS, and one minor QTL on 6BS had significant effects in reducing stripe rust severity. The QTL were validated using composite interval mapping (CIM) and inclusive composite interval mapping (ICIM). These methods explained 59.0%–74.1% of the phenotype variation in disease response. The QTL on chromosome 1BL was confirmed to beYr29/Lr46and the one on 3BS was the resistance allele identified in CIMMYT germplasm but was notYr30/Sr2. The QTL on 6BS probably corresponded to previously known QTL. RILs with combined QTL were more resistant than those with single or no QTL. Kompetitive allele-specific PCR (KASP) assays for the QTL with largest effect QTL on chromosome 3BS were performed on a subset of RILs and 150 unrelated wheat lines. The QTL on 3BS with its linked KASP markers can be used in marker-assisted selection to improve stripe rust resistance in breeding programs.
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影响因子：
5.4
作者：
Rosewarne, G. M.;Herrera-Foessel, S. A.;Singh, R. P.;Huerta-Espino, J.;Lan, C. X.;He, Z. H.
通讯作者：
He, Z. H.
DOI：
10.1071/9780643101463
发表时间：
1995
期刊：
--
影响因子：
--
作者：
R. McIntosh;C. Wellings;R. Park
通讯作者：
R. McIntosh;C. Wellings;R. Park
影响因子：
5.4
作者：
Herrera-Foessel, Sybil A.;Singh, Ravi P.;Lagudah, Evans S.
通讯作者：
Lagudah, Evans S.
DOI：
10.3724/sp.j.1006.2009.00239
发表时间：
2009-01-01
期刊：
Acta Agronomica Sinica
影响因子：
--
作者：
Wang JianKang, Wang JianKang;Wang, J. K.
通讯作者：
Wang, J. K.
影响因子：
3.1
作者：
Xiaofei Zhang;M. Rouse;I. C. Nava;Yue Jin;J. Anderson
通讯作者：
Xiaofei Zhang;M. Rouse;I. C. Nava;Yue Jin;J. Anderson