Overexpression of IlHMA2, from Iris lactea, Improves the Accumulation of and Tolerance to Cadmium in Tobacco.
Overexpression of IlHMA2, from Iris lactea, Improves the Accumulation of and Tolerance to Cadmium in Tobacco.
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DOI：
10.3390/plants12193460
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发表时间：
2023-09-30
期刊：
影响因子：
--
通讯作者：
Guo Q
中科院分区：
文献类型：
--
作者：
Li C;Wang Q;Hou X;Zhao C;Guo Q
镉从根到地的长距离运输是镉植物修复的关键因素。我们以前的研究表明，重金属P1B2-ATPase，IlHMA2，通过介导Cd的长途运输促进了Cd的积累，有助于Cd积累植物的修复。然而，IlHMA2基因的过表达是否能提高植物对Cd的积累和耐性，目前还不清楚。在本研究中，我们获得了高表达IlHMA2的转基因烟草，并检测了其在50 mg/L Cd胁迫下对Cd和锌的运转和积累以及生理生化特性的影响。与野生型相比，过表达IlHMA2显著增加了木质部汁液中Cd的浓度，导致了Cd从根到冠的转运。同时，过量表达IlHMA2促进了锌的积累，同时提高了抗氧化酶(SOD、POD和CAT)的活性和脯氨酸含量，从而减少了ROS的过量生产。这些证据表明，较高的锌浓度和较低的ROS水平可以极大地缓解转基因烟草的镉毒害，从而提高烟草的生长和耐性。总之，过表达IlHMA2基因可以促进Cd的积累，提高烟草对Cd污染的耐受性。这将为提高Cd的植物修复效率提供有价值的参考。
Long-distance transport cadmium (Cd) from roots to shoots is a key factor for Cd phytoremediation. Our previous study indicated that heavy metal P1B2-ATPases, IlHMA2, was involved in improving the accumulation of Cd via mediated long-distance transport Cd, contributing to the phytoremediation in Cd accumulator Iris lactea. However, whether the overexpression of IlHMA2 could enhance the accumulation and tolerance to Cd remains unclear in plants. Here, we generated transgenic tobacco overexpressing IlHMA2 and tested its effect on the translocation and accumulation of Cd and zinc (Zn), as well as the physio-biochemical characteristics under 50 mg/L Cd exposure. The overexpression of IlHMA2 significantly increased Cd concentrations in xylem saps, resulting in enhanced root-to-shoot Cd translocation compared with wild-type. Meanwhile, overexpressing IlHMA2 promoted Zn accumulations, accompanied by elevating proline contents and antioxidant enzyme activity (SOD, POD, and CAT) to diminish the overproduction of ROS in transgenic tobacco. These pieces of evidence suggested that higher Zn concentrations and lower ROS levels could tremendously alleviate Cd toxicity for transgenic tobacco, thereby improving the growth and tolerance. Overall, the overexpression of IlHMA2 could facilitate Cd accumulation and enhance its tolerance in tobacco exposed to Cd contaminations. This would provide a valuable reference for improving Cd phytoremediation efficiency.
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