Bioluminescent Probes for Imaging Biology beyond the Culture Dish.
Bioluminescent Probes for Imaging Biology beyond the Culture Dish.
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DOI:
10.1021/acs.biochem.7b00435
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发表时间:
2017-10-03
期刊:
影响因子:
2.9
通讯作者:
Prescher JA
中科院分区:
文献类型:
--
作者:
Rathbun CM;Prescher JA
Bioluminescence with luciferase-luciferin pairs is an attractive method for surveying cells in live tissues and whole organisms. Recent advances in luciferin chemistry and luciferase engineering are further expanding the scope of the technology. It is now possible to spy on cells in a variety of deep tissues and visualize multicellular interactions—feats that are enabling new questions to be asked and new ideas to be explored. This perspective piece highlights recent successes in bioluminescent probe development and their applications to imaging in live cells, tissues, and animals.
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