Controlling Microbial Dynamics through Selective Solute Transport across Functional Nanocultures
Controlling Microbial Dynamics through Selective Solute Transport across Functional Nanocultures
复制标题
通过功能性纳米培养物的选择性溶质运输控制微生物动力学
DOI：
10.1021/acsapm.1c01422
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发表时间：
2022
影响因子：
5
通讯作者：
Niepa, Tagbo H.
中科院分区：
文献类型：
--
作者：
Davidson, Shanna-Leigh;Niepa, Tagbo H.
对微生物动力学评估工具的需求使得细胞培养技术的小型化和微系统的设计成为必要，这些微系统有助于在明确定义的环境中对微生物进行询问。正如这项工作所描述的那样，纳米培养就是这样一种评估工具：使用流动聚焦微流体装置产生的纳米升大小的微胶囊，以高通量的方式隔离和培养微生物。通过操纵聚合物外壳的化学性质，可以设计纳米培养物来实现功能，例如选择性渗透性，促进代谢物和其他控制细胞生长和表征群落动态所必需的小分子的运输。在这项工作中，通过研究与控制细胞动力学相关的选择分子的扩散，包括抗菌剂、荧光染色探针和糖，研究了以n， n -二甲基烯丙胺（DMAA）为功能化的聚（二甲基硅氧烷）基膜的运输特性。此外，Flory-Huggins相互作用参数被评估为一种预测工具，以阐明所选分子在纳米培养物中的分配和运输。通过产生含有大肠杆菌的纳米培养物，实验证实了分子的扩散，细胞生长被用作确定成功分子扩散的代理。在我们的研究中，我们确定了Flory-Huggins相互作用参数可以准确地预测分子子集在PDMS膜上的扩散，特别是那些相互作用参数低于指定临界阈值的分子。然而，随着相互作用参数的增加，预测变得不那么准确。总的来说，这些发现将为我们理解如何有效地利用纳米培养来研究天然和合成群落中复杂的协同和拮抗微生物行为铺平道路，目的是更好地模拟自然微环境，并增加与复杂微生物群落相关的未知分子的发现。
The need for assessment tools for microbial dynamics has necessitated the miniaturization of cell-culturing techniques, and the design of microsystems that facilitate the interrogation of microorganisms in-well-defined environments. The nanocultures, as described in this work, are such an assessment tool: nanoliter-sized microcapsules generated using a flow-focusing microfluidic device to sequester and cultivate microbes in a high-throughput manner. By manipulating the chemistry of their polymeric shell, the nanocultures can be designed to achieve functionalities, such as selective permeability, facilitating the transport of metabolites and other small molecules essential to control cell growth and to characterize community dynamics. In this work, the transport properties of a poly(dimethylsiloxane)-based membrane functionalized withN,N-dimethylallylamine (DMAA) have been examined by investigating the diffusion of selected molecules relevant to controlling cell dynamics, including antimicrobials, fluorescent staining probes, and sugars. Furthermore, the Flory–Huggins interaction parameter was evaluated as a predictive tool to elucidate the partitioning and transport of selected molecules into the nanocultures. Diffusion of molecules was confirmed experimentally by generating nanocultures containingEscherichia colicells, whereby cell growth was used as a proxy for determination of successful molecule diffusion. In our study, we determined that the Flory–Huggins interaction parameters can accurately predict the diffusion of a subset of molecules across PDMS membrane, notably, those with an interaction parameter below a designated critical threshold. However, the prediction becomes less accurate as interaction parameters increased. Overall, these findings will pave the way in our understanding of effectively using the nanocultures to study complex synergistic and antagonistic microbial behaviors in both natural and synthetic communities, with the goal of better simulating natural microenvironments and increasing discoverability of unknown molecules that are relevant to complex microbial communities.
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影响因子：
3.7
作者：
Da;Ching;C. Chu;Hsiu
通讯作者：
Hsiu
DOI：
10.1073/pnas.76.12.6040
发表时间：
1979
影响因子：
11.1
作者：
J. Hildebrand
通讯作者：
J. Hildebrand
影响因子：
16.6
作者：
Ramijan, Karina;Ultee, Eveline;Claessen, Dennis
通讯作者：
Claessen, Dennis
影响因子：
5.8
作者：
Stefanis, Emmanuel;Panayiotou, Costas
通讯作者：
Panayiotou, Costas
影响因子：
9.7
作者：
Borde, Annika;Larsson, Mikael;Larsson, Anette
通讯作者：
Larsson, Anette