Effects of maternal dexamethasone on expression of SP-A, SP-B, and SP-C in the fetal rat lung.
Effects of maternal dexamethasone on expression of SP-A, SP-B, and SP-C in the fetal rat lung.
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母体地塞米松对胎鼠肺中 SP-A、SP-B 和 SP-C 表达的影响。
DOI：
10.1165/ajrcmb/4.4.304
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发表时间：
1991
影响因子：
6.4
通讯作者：
Shannon,JM
中科院分区：
文献类型：
--
作者：
Schellhase,DE;Shannon,JM
产前给予糖皮质激素已被证明能提高胎儿表面活性剂的产生。由于表面活性剂蛋白在表面活性剂的功能和分泌中起重要作用，我们希望确定母体糖皮质激素给药对其胎儿表达和外观的影响。在妊娠第14 ~ 16天或第16天给予定时妊娠大鼠地塞米松（DEX）（1 mg/kg/天）或0.9%生理盐水，第17天（足月第22天）牺牲，妊娠第14 ~ 18天，或第16 ~ 18天，或第18天牺牲，第19天牺牲。采用酶联免疫吸附法测定经处理和对照的雄性和雌性胎鼠肺匀浆中sp的含量。通过Northern blot分析，还测定了治疗组和对照组雄性和雌性胎鼠肺中SP-A、SP-B和SP-C mrna的丰度。在第17天处死的窝鼠中，在第14 ~ 16天和第16天给予DEX， SP-A含量显著增加。SP-A mRNA的表达在第17天的对照组胎儿中未被检测到，但在DEX治疗1或3天后，SP-A mRNA的表达变得明显。在DEX治疗1或3天后，SP-B和SP-C的mrna丰度也在第17天的胎儿中增加。在第19天处死的窝鼠中，在第14 ~ 18天和第16 ~ 18天施用DEX可显著提高SP-A含量；DEX处理第18天对sp含量无影响。有趣的是，无论DEX治疗时间长短，SP-A mRNA的丰度在第19天的胎儿肺中没有显著变化。然而，在先前的DEX治疗3或5天后，SP-B和SP-C的mrna丰度在第19天的胎儿肺中显著增加。在第17天或第19天，胎儿性别对dex处理或对照动物SP-A、SP-B和SP-C mrna的含量和丰度没有影响。这些结果表明，母体DEX治疗对SP-A和SP-A mRNA的积累有不同的影响，这取决于胎儿肺发育阶段和治疗时间。然而，母体DEX对SP-B和SP-C mrna的影响似乎与胎儿肺发育阶段和治疗时间无关，这表明在发育中的大鼠肺中存在表面活性剂蛋白基因的差异调控。
Prenatal administration of glucocorticoids has been shown to enhance surfactant production in the fetus. Since the surfactant proteins play an important role in surfactant function and secretion, we wished to determine the effects of maternal glucocorticoid administration on their fetal expression and appearance. Daily dexamethasone (DEX)(1 mg/kg/day) or 0.9% saline was administered to timed-pregnant rats on gestational days 14 through 16 or on day 16 with sacrifice on day 17 (term day 22), and on gestational days 14 through 18, or days 16 through 18, or day 18 with sacrifice on day 19. SP-Acontent was determined in lung homogenates from treated and control male and female fetal rats by an enzyme-linked immunosorbent assay. The abundance of mRNAs for SP-A, SP-B, and SP-C per fixed amount of total cellular RNA was also determined in lungs from treated and control male and female fetal rats by Northern blot analysis.In litters sacrificed on day 17, DEX administered on days 14 through 16 and on day 16 resulted in significant increases in SP-A content. Expression of SP-A mRNA, which was not detectable in control fetuses on day 17, became clearly apparent after either 1or 3 d of DEX treatment. The abundance of mRNAs for SP-B and SP-C also increased in day-17 fetuses after either 1 or 3 d of DEX treatment. In litters sacrificed on day 19, DEX administered on days 14 through 18 and on days 16 through 18 resulted in significant increases in SP-A content; treatment with DEX on day 18 had no effect on SP-Acontent. Interestingly, the abundance of SP-A mRNA did not change significantly in day-19 fetal lungs regardless of the duration of DEX treatment. The abundance of mRNAs for SP-B and SP-C, however, significantly increased in day-19 fetal lungs after 3 or 5 d of prior DEX treatment. Fetal sex had no effect on SP-Acontent and abundance of mRNAs for SP-A, SP-B, and SP-C in DEX-treated or control animals on either day 17 or 19. These findings suggest that maternal DEX treatment has different effects on SP-A and SP-A mRNA accumulation, depending on the stage of fetal lung development and duration of treatment. The effectsof maternal DEX on mRNAs for SP-B and SP-C, however, appear to be independent of the stage of fetal lung development and duration of treatment, suggesting differential regulation of the surfactant protein genes in the developing rat lung.
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皮质类固醇对胎鼠和新生大鼠肺和肝中糖原含量和类固醇11-还原酶活性的影响。
DOI：
10.1159/000241600
发表时间：
1982
期刊：
Biology of the neonate
影响因子：
--
作者：
Smith Bt;Tanswell Ak;D. Minshall;W. N. Bogues;E. Vreeken
通讯作者：
E. Vreeken
糖皮质激素对主要表面活性剂相关蛋白（SP-A）及其信使核糖核酸的调节以及人胎肺体外形态发育的调节。
影响因子：
4.8
作者：
Odom,MJ;Snyder,JM;Boggaram,V;Mendelson,CR
通讯作者：
Mendelson,CR
肺表面活性剂脱辅基蛋白A结构和大鼠肺泡II型细胞表面活性剂分泌的调节。
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kuroki,Y;Mason,RJ;Voelker,DR
通讯作者：
Voelker,DR
胎儿和新生大鼠体内地塞米松治疗后 35kDa 表面活性剂相关蛋白及其 mRNA 增加
影响因子：
2.9
作者：
D. Phelps;S. Church;S. Kourembanas;H. Taeusch;J. Floros
通讯作者：
J. Floros
影响因子：
2.9
作者：
DURNAM, DM;PALMITER, RD
通讯作者：
PALMITER, RD