Whole-genome analysis of the colonization-resistant bacterium Phytobacter sp. SCO41T isolated from Bacillus nematocida B16-fed adult Caenorhabditis elegans
Whole-genome analysis of the colonization-resistant bacterium Phytobacter sp. SCO41T isolated from Bacillus nematocida B16-fed adult Caenorhabditis elegans
复制标题
定植抗性细菌植物杆菌的全基因组分析。
DOI：
10.1007/s11033-018-04574-w
复制
发表时间：
2019-03
影响因子：
2.8
通讯作者：
Qiuhong Niu
中科院分区：
文献类型：
--
作者：
Bowen Wang;Bingfen Huang;Junmei Chen;Wenpeng Li;Ling Yang;Yunguang Yao;Qiuhong Niu
定殖抗性是细菌与宿主相互作用的重要属性，但其机制仍不完全清楚。本研究发现植物杆菌SCO41T能有效抑制杀线虫芽孢杆菌B16在秀丽隐杆线虫体内的定殖，并揭示了其定殖抗性机制。从自由生活线虫C.从腐烂的水果和土壤中提取的秀丽线虫。通过对其中一个菌株的基本特征和基因组图谱的分析，探讨了该菌株的定殖抗性机制。优美的此外，我们进行了外源性铁补充和基因簇敲除实验，以验证测序结果。结果表明，3株菌亲缘关系较近，初步鉴定为植杆菌属。模式菌株为SCO41T（= CICC 24103T= KCTC 52362T）。全基因组分析表明，csgA、csgB、csgC、csgE、csgF和csgG参与了卷曲粘附过程，fepA、fepB、fepC、fepD和fepG在SCO41 T抗B定殖中起重要作用。杀线虫剂B16在C.通过竞争铁元素来获得营养。外源铁的补充可以增加B的定殖。杀线虫剂B16，其另外通过缺失突变株确认。csg基因家族参与了SCO41Tin C的定殖。优雅的Curli可能有助于SCO41Tin C的定殖。肠杆菌素在SCO41 T中起关键作用，可抵抗B的定植。杀线虫剂B16通过竞争铁。
Colonization resistance is an important attribute for bacterial interactions with hosts, but the mechanism is still not completely clear. In this study, we found that Phytobacter sp. SCO41Tcan effectively inhibit the in vivo colonization of Bacillus nematocida B16 in Caenorhabditis elegans, and we revealed the colonization resistance mechanism. Three strains of colonization-resistant bacteria, SCO41T, BX15, and BC7, were isolated from the intestines of the free-living nematode C. elegans derived from rotten fruit and soil. The primary characteristics and genome map of one of the three isolates was investigated to explore the underlying mechanism of colonization resistance in C. elegans. In addition, we performed exogenous iron supplementation and gene cluster knockout experiments to validate the sequencing results. The results showed that relationship was close among the three strains, which was identified as belonging to the genus Phytobacter. The type strain is SCO41T(= CICC 24103T= KCTC 52362T). Whole genome analysis showed that csgA, csgB, csgC, csgE, csgF, and csgG were involved in the curli adhesive process and that fepA, fepB, fepC, fepD, and fepG played important roles in SCO41Tagainst the colonization of B. nematocida B16 in C. elegans by competing for iron. Exogenous iron supplementation showed that exogenous iron can increase the colonization of B. nematocida B16, which was additionally confirmed by a deletion mutant strain. The csg gene family contributes to the colonization of SCO41Tin C. elegans. Curli potentially contribute to the colonization of SCO41Tin C. elegans, and enterobactin has a key role in SCO41Tto resist the colonization of B. nematocida B16 by competing for iron.
登录
查看更多内容
杀线虫芽孢杆菌 B16 感染后秀丽隐杆线虫肠道菌群的变化
影响因子：
4.6
作者：
Niu Q;Zhang L;Zhang K;Huang X;Hui F;Kan Y;Yao L
通讯作者：
Yao L
影响因子：
6.7
作者：
Kai-Larsen Y;Lüthje P;Chromek M;Peters V;Wang X;Holm A;Kádas L;Hedlund KO;Johansson J;Chapman MR;Jacobson SH;Römling U;Agerberth B;Brauner A
通讯作者：
Brauner A
DOI：
10.1038/nri3535
发表时间：
2013-11
期刊：
Nature reviews. Immunology
影响因子：
--
作者：
通讯作者：
--
影响因子：
5.1
作者：
Saldaña Z;Xicohtencatl-Cortes J;Avelino F;Phillips AD;Kaper JB;Puente JL;Girón JA
通讯作者：
Girón JA
DOI：
10.1007/s10482-008-9222-6
发表时间：
2008-02
期刊：
Antonie van Leeuwenhoek
影响因子：
--
作者：
Y. Momose;K. Hirayama;K. Itoh
通讯作者：
Y. Momose;K. Hirayama;K. Itoh