Potential biomarkers of infertility associated with microbiome imbalances.
Potential biomarkers of infertility associated with microbiome imbalances.
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DOI：
10.1111/aji.13438
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发表时间：
2021-10
期刊：
影响因子：
--
通讯作者：
Gutiérrez G
中科院分区：
文献类型：
--
作者：
Azpiroz MA;Orguilia L;Palacio MI;Malpartida A;Mayol S;Mor G;Gutiérrez G
本研究的目的是探讨阴道/直肠微生物群失衡和miRNA表达与不孕症的可能关系。观察性、探索性、初步研究。共纳入287例多次体外受精失败的不孕症患者。20名有生育能力的妇女，没有试管受精失败，作为对照组。拭子样本从阴道和直肠采集。检测阴道和直肠组织中NGS的微生物组成和实时荧光定量聚合酶链式反应(Real-Time-PCR)中miRNA的表达。分析血液(胰岛素、维生素D、低密度脂蛋白-胆固醇、抗核抗体、TPO、甘油三酯和ASCA抗体)和唾液(SIgA)的免疫代谢指标。与生育组相比，不孕症患者直肠水平的细菌丰富度较低，菲米米特/类杆菌比例较高，阴道样本中的短乳杆菌/乳杆菌比例较高。在同一直肠拭子样本中，我们发现与紧密连接中断和酵母过度生长相关的miR-21-5p在不明原因不孕症组中上调，而与炎症相关的miR-155-5p过度表达(*p<；0.05)。这些解除调控的miRNAs也在同一患者的阴道样本中上调(P<；0.05)。MiRNAs可能是微生物群失衡对不明原因不孕妇女炎症影响的潜在生物标记物。
The aim of this study was to investigate the possible relationship between vaginal/rectal microbiome disbalances and miRNA expression with infertility. Observational, exploratory, preliminary study. A total of 287 multiple IVF failure infertile patients were recruited. Twenty fertile women, not IVF failure, were recruited as the control group. Swab samples were collected from the vagina and rectum. Microbial composition by NGS and miRNA expression by real-time PCR of vaginal and rectal samples was measured. Immunometabolic markers from blood (insulin, vitamin D, LDL-cholesterol, ANA, TPO, Tg, and ASCA antibodies) and saliva (sIgA) were analyzed. Infertile patients showed a lower bacterial richness and increased Firmicutes/Bacteroidetes ratio at rectal level and an increased Lactobacillus brevis/Lactobacillus iners ratio in vaginal samples regarding the fertile group. In the same rectal swab samples, we found that miR-21–5p, which is associated with tight junction disruption and yeast overgrowth, is upregulated and that miR-155–5p, which is associated with inflammation, is overexpressed in the unexplained infertile group (*p < .05). These deregulated miRNAs were also upregulated in the vaginal samples from the same patients (*p < .05). miRNAs could be potential biomarkers of the inflammatory impact of microbiome disbalances in unexplained infertile women.
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25.7
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