Cardiac Derived CD51-Positive Mesenchymal Stem Cells Enhance the Cardiac Repair Through SCF-Mediated Angiogenesis in Mice With Myocardial Infarction.
Cardiac Derived CD51-Positive Mesenchymal Stem Cells Enhance the Cardiac Repair Through SCF-Mediated Angiogenesis in Mice With Myocardial Infarction.
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心脏来源的 CD51 阳性间充质干细胞通过 SCF 介导的血管生成增强心肌梗塞小鼠的心脏修复
DOI：
10.3389/fcell.2021.642533
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发表时间：
2021
影响因子：
5.5
通讯作者：
Jiang MH
中科院分区：
文献类型：
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作者：
Xie DM;Chen Y;Liao Y;Lin W;Dai G;Lu DH;Zhu S;Yang K;Wu B;Chen Z;Peng C;Jiang MH
目的：许多组织中含有可促进组织止血和修复的间充质基质/干细胞（MSCs）。然而，没有典型的标志物来识别常驻心脏间充质干细胞。我们的目的是确定CD51是否可以作为心脏间充质干细胞的最佳标记物，并评估其对急性心肌梗死（AMI）小鼠的治疗潜力。方法：采用流式细胞术从C57BL/6小鼠（7日龄）中分离心源性CD51+ CD31-CD45-Ter119细胞（CD51+cMSCs）。CD51+cMSCs具有增殖能力、多向分化潜力和典型msc相关标志物的表达等特征。成年C57BL/6小鼠（12周龄）通过永久结扎左冠状动脉前降支建立AMI模型。通过超声心动图和病理染色评估CD51+cMSCs的治疗效果。为了确定潜在的机制，使用慢病毒敲低CD51+cMSCs的基因（干细胞因子[SCF]）表达。结果：在本研究中，CD51在小鼠心内膜、心外膜、心肌等心壁全层均有表达，且随年龄增长表达降低。重要的是，CD51+cMSCs在体外具有强大的自我更新潜力和多谱系分化能力，并表达典型的msc相关表面蛋白。此外，CD51+cMSC移植可显著改善小鼠心肌梗死后的心功能，并通过促血管生成活性减轻心肌纤维化。此外，CD51+cMSCs分泌的SCF在体内和体外血管生成中都发挥了重要作用。结论：总的来说，CD51是一种新的心脏驻留MSCs标记物，CD51+cMSC治疗至少部分通过scf介导的血管生成增强心脏修复。
Objective: Many tissues contained resident mesenchymal stromal/stem cells (MSCs) that facilitated tissue hemostasis and repair. However, there is no typical marker to identify the resident cardiac MSCs. We aimed to determine if CD51 could be an optimal marker of cardiac MSCs and assess their therapeutic potential for mice with acute myocardial infarction (AMI). Methods: Cardiac-derived CD51+CD31–CD45–Ter119– cells (named CD51+cMSCs) were isolated from C57BL/6 mice(7-day-old) by flow cytometry. The CD51+cMSCs were characterized by proliferation capacity, multi-differentiation potential, and expression of typical MSC-related markers. Adult C57BL/6 mice (12-week-old) were utilized for an AMI model via permanently ligating the left anterior descending coronary artery. The therapeutic efficacy of CD51+cMSCs was estimated by echocardiography and pathological staining. To determine the underlying mechanism, lentiviruses were utilized to knock down gene (stem cell factor [SCF]) expression of CD51+cMSCs. Results: In this study, CD51 was expressed in the entire layers of the cardiac wall in mice, including endocardium, epicardium, and myocardium, and its expression was decreased with age. Importantly, the CD51+cMSCs possessed potent self-renewal potential and multi-lineage differentiation capacity in vitro and also expressed typical MSC-related surface proteins. Furthermore, CD51+cMSC transplantation significantly improved cardiac function and attenuated cardiac fibrosis through pro-angiogenesis activity after myocardial infarction in mice. Moreover, SCF secreted by CD51+cMSCs played an important role in angiogenesis both in vivo and in vitro. Conclusions: Collectively, CD51 is a novel marker of cardiac resident MSCs, and CD51+cMSC therapy enhances cardiac repair at least partly through SCF-mediated angiogenesis.
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影响因子：
120.7
作者：
Heldman, Alan W.;DiFede, Darcy L.;Fishman, Joel E.;Zambrano, Juan P.;Trachtenberg, Barry H.;Karantalis, Vasileios;Mushtaq, Muzammil;Williams, Adam R.;Suncion, Viky Y.;McNiece, Ian K.;Ghersin, Eduard;Soto, Victor;Lopera, Gustavo;Miki, Roberto;Willens, Howard;Hendel, Robert;Mitrani, Raul;Pattany, Pradip;Feigenbaum, Gary;Oskouei, Behzad;Byrnes, John;Lowery, Maureen H.;Sierra, Julio;Pujol, Mariesty V.;Delgado, Cindy;Gonzalez, Phillip J.;Rodriguez, Jose E.;Bagno, Luiza Lima;Rouy, Didier;Altman, Peter;Foo, Cheryl Wong Po;da Silva, Jose;Anderson, Erica;Schwarz, Richard;Mendizabal, Adam;Hare, Joshua M.
通讯作者：
Hare, Joshua M.
影响因子：
9.2
作者：
Liang J;Wu YL;Chen BJ;Zhang W;Tanaka Y;Sugiyama H
通讯作者：
Sugiyama H
影响因子：
2.6
作者：
Covas, Dimas T.;Panepucci, Rodrigo A.;Zago, Marco A.
通讯作者：
Zago, Marco A.
DOI：
10.1073/pnas.1220185110
发表时间：
2013-02-19
影响因子：
11.1
作者：
Gomes, Samirah A.;Rangel, Erika B.;Hare, Joshua M.
通讯作者：
Hare, Joshua M.
巢蛋白阳性干间质细胞作为治疗睾丸间质细胞功能障碍的潜在来源的表征。
影响因子：
44.1
作者：
通讯作者：
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