Berberine Suppresses Adipocyte Differentiation via Decreasing CREB Transcriptional Activity.
Berberine Suppresses Adipocyte Differentiation via Decreasing CREB Transcriptional Activity.
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小檗碱通过降低 CREB 转录活性抑制脂肪细胞分化
DOI：
10.1371/journal.pone.0125667
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Zhou L
中科院分区：
文献类型：
--
作者：
Zhang J;Tang H;Deng R;Wang N;Zhang Y;Wang Y;Liu Y;Li F;Wang X;Zhou L
Berberine, one of the major constituents of Chinese herb Rhizoma coptidis, has been demonstrated to lower blood glucose, blood lipid, and body weight in patients with type 2 diabetes mellitus. The anti-obesity effect of berberine has been attributed to its anti-adipogenic activity. However, the underlying molecular mechanism remains largely unknown. In the present study, we found that berberine significantly suppressed the expressions of CCAAT/enhancer-binding protein (C/EBP)α, peroxisome proliferators-activated receptor γ2 (PPARγ2), and other adipogenic genes in the process of adipogenesis. Berberine decreased cAMP-response element-binding protein (CREB) phosphorylation and C/EBPβ expression at the early stage of 3T3-L1 preadipocyte differentiation. In addition, CREB phosphorylation and C/EBPβ expression induced by 3-isobutyl-1-methylxanthine (IBMX) and forskolin were also attenuated by berberine. The binding activities of cAMP responsive element (CRE) stimulated by IBMX and forskolin were inhibited by berberine. The binding of phosphorylated CREB to the promoter of C/EBPβ was abrogated by berberine after the induction of preadipocyte differentiation. These results suggest that berberine blocks adipogenesis mainly via suppressing CREB activity, which leads to a decrease in C/EBPβ-triggered transcriptional cascades.
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影响因子：
5.7
作者：
Vingtdeux, Valerie;Chandakkar, Pallavi;Marambaud, Philippe
通讯作者：
Marambaud, Philippe
DOI：
10.1006/bbrc.1997.7627
发表时间：
1997-11-07
影响因子：
3.1
作者：
Clarke, SL;Robinson, CE;Gimble, JM
通讯作者：
Gimble, JM
影响因子：
6.5
作者：
Lee, Joo-Won;Choe, Sung Sik;Kim, Jae Bum
通讯作者：
Kim, Jae Bum
影响因子：
5.3
作者：
Petersen, Rasmus Koefoed;Madsen, Lise;Kristiansen, Karsten
通讯作者：
Kristiansen, Karsten
影响因子：
5.6
作者：
Kim, Eun Jin;Lee, Dong Hun;Yoon, Do Young
通讯作者：
Yoon, Do Young