Response of denitrifying genes coding for nitrite (nirK or nirS) and nitrous oxide (nosZ) reductases to different physico-chemical parameters during agricultural waste composting
Response of denitrifying genes coding for nitrite (nirK or nirS) and nitrous oxide (nosZ) reductases to different physico-chemical parameters during agricultural waste composting
复制标题
编码亚硝酸盐（nirK或nirS）和一氧化二氮（nosZ）还原酶的反硝化基因对农业废物堆肥过程中不同理化参数的响应
DOI：
10.1007/s00253-014-6293-3
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发表时间：
2015-01
影响因子：
5
通讯作者：
He Ling
中科院分区：
文献类型：
--
作者：
Zhang Lihua;Zeng Guangming;Zhang Jiachao;Chen Yaoning;Yu Man;Lu Lunhui;Li Hui;Zhu Yuan;Yuan Yujie;Huang Aizhi;He Ling
通过实时定量PCR（qPCR）测定基因丰度。通过回归分析，分析了理化参数与各基因丰度之间的相关性。qPCR结果显示，nosZ基因的丰度高于nirK和nirS基因。nirK基因的丰度高于nirS基因，表明nirK基因编码的含铜酶亚硝酸盐还原剂在亚硝酸盐还原过程中的作用大于nirS基因编码的细胞色素cd 1亚硝酸盐还原酶亚硝酸盐还原剂。回归分析表明：（1）nirK基因丰度与堆温呈二次回归模型相关;（2）nirS基因丰度与堆温和NH 4+浓度呈线性相关，与NO3-浓度和pH呈二次回归模型相关;（3）nosZ基因丰度与pH呈二次回归模型相关，与水溶性碳（WSC）呈线性相关。
The genes abundances were determined by real-time quantitative PCR (qPCR). The correlations between physico-chemical parameters and denitrifying genes abundances were analysed by regression analysis. qPCR results showed that the nosZ gene abundance was higher than that of nirK and nirS genes. The nirK gene abundance was higher than nirS gene indicating that nitrite reducers with Cu-containing enzyme encoded by nirK gene were more of importance than those with cytochrome cd1 nitrite reductase encoded by nirS gene in the nitrite reduction step. Regression analysis suggested that (1) nirK gene abundance was correlated with pile temperature following quadratic model; (2) nirS gene abundance was linearly correlated with pile temperature and concentration of NH4+, while correlated with concentration of NO3− and pH following inverse and quadratic model respectively; (3) nosZ gene abundance was quadratically correlated with pH and linearly correlated with water soluble carbon (WSC).
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DOI：
10.1007/978-3-662-08724-4_20
发表时间：
2002
期刊：
--
影响因子：
--
作者：
S. M. Tiquia
通讯作者：
S. M. Tiquia
影响因子：
4.4
作者：
S. Hallin;P. Lindgren
通讯作者：
S. Hallin;P. Lindgren
DOI：
10.1017/s0021859699007625
发表时间：
2000-05
期刊：
The Journal of Agricultural Science
影响因子：
--
作者：
S. Sommer;H. Møller
通讯作者：
S. Sommer;H. Møller
影响因子：
6.5
作者：
Gödde, M;Conrad, R
通讯作者：
Conrad, R
影响因子：
4.4
作者：
Kandeler, Ellen;Deiglmayr, Kathrin;Philippot, Laurent
通讯作者：
Philippot, Laurent