Characterization of microbiota composition and presence of selected antibiotic resistance genes in carriage water of ornamental fish.
Characterization of microbiota composition and presence of selected antibiotic resistance genes in carriage water of ornamental fish.
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DOI：
10.1371/journal.pone.0103865
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Rychlik I
中科院分区：
文献类型：
--
作者：
Gerzova L;Videnska P;Faldynova M;Sedlar K;Provaznik I;Cizek A;Rychlik I
观赏鱼的国际贸易逐渐被认为是各种不同抗生素耐药细菌的重要来源。因此，在这项研究中，我们表征了来自3个不同大洲的观赏鱼运输水中发现的微生物群中选定的抗生素耐药基因的流行情况。实时荧光定量PCR结果显示，sul1基因在100个细菌中有11个存在。每100个细菌中有6个存在tet(A)，每100个细菌中有1-2个存在strA、tet(G)、sul2和aadA。I类整合子在运载水微生物群中非常普遍，然而，焦磷酸测序显示，I类整合子中仅存在12种不同的抗生素基因盒。16S rRNA基因V3/V4可变区测序鉴定的菌群包括变形菌门（48%）、拟杆菌门（29.5%）、厚壁菌门（17.8%）、放线菌门（2.1%）和梭杆菌门（1.6%）。细菌培养验证的抗生素耐药基因流行率与菌群组成的相关性分析表明，在α -、β -和γ -变形菌中存在sul1 - sul2、tet(A)、tet(B)、tet(G)、cat、cml、bla、strA、aacA、aph和aadA的主要宿主，其中肠杆菌科、假单胞菌科、根瘤菌科和Comamonadaceae代表与所测抗生素耐药基因呈正相关。
International trade with ornamental fish is gradually recognized as an important source of a wide range of different antibiotic resistant bacteria. In this study we therefore characterized the prevalence of selected antibiotic resistance genes in the microbiota found in the carriage water of ornamental fish originating from 3 different continents. Real-time PCR quantification showed that the sul1 gene was present in 11 out of 100 bacteria. tet(A) was present in 6 out of 100 bacteria and strA, tet(G), sul2 and aadA were present in 1–2 copies per 100 bacteria. Class I integrons were quite common in carriage water microbiota, however, pyrosequencing showed that only 12 different antibiotic gene cassettes were present in class I integrons. The microbiota characterized by pyrosequencing of the V3/V4 variable region of 16S rRNA genes consisted of Proteobacteria (48%), Bacteroidetes (29.5%), Firmicutes (17.8%), Actinobacteria (2.1%) and Fusobacteria (1.6%). Correlation analysis between antibiotic resistance gene prevalence and microbiota composition verified by bacterial culture showed that major reservoirs of sul1 sul2, tet(A), tet(B) tet(G), cat, cml, bla, strA, aacA, aph and aadA could be found among Alpha-, Beta- and Gammaproteobacteria with representatives of Enterobacteriaceae, Pseudomonadaceae, Rhizobiaceae and Comamonadaceae being those most positively associated with the tested antibiotic resistance genes.
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影响因子：
56.9
作者：
Venter, JC;Remington, K;Smith, HO
通讯作者：
Smith, HO
影响因子：
3.3
作者：
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影响因子：
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作者：
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