The value of DCE-MRI in assessing histopathological and molecular biological features in induced rat epithelial ovarian carcinomas.
The value of DCE-MRI in assessing histopathological and molecular biological features in induced rat epithelial ovarian carcinomas.
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DCE-MRI评估诱导性大鼠上皮性卵巢癌组织病理学和分子生物学特征的价值
DOI：
10.1186/s13048-017-0362-z
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发表时间：
2017-09-26
影响因子：
4
通讯作者：
Li RK
中科院分区：
文献类型：
--
作者：
Yuan SJ;Qiao TK;Qiang JW;Cai SQ;Li RK
目的探讨动态增强磁共振成像（DCE-MRI）对诱发大鼠卵巢上皮癌（EOCs）的组织病理学和分子生物学特征的评价。用7，12-二甲基苯并[A]蒽（DMBA）诱导46只SD大鼠原位EOCs。常规MRI和DCE-MRI检查肿瘤的形态学和灌注特征，包括时间-信号强度曲线（TIC）、容积传递常数（Ktranss）、速率常数（Kep）、血管外细胞外间隙容积比（Ve）和初始曲线下面积（IAUC）。DCE-MRI参数与组织学分级、微血管密度（MVD）、血管内皮生长因子（VEGF）、Ki 67阳性细胞比例及血清癌抗原125（CA 125）水平相关。46只大鼠中有35只发生EOC。DCE-MRI显示EOC中III型TIC比II型更常见（29/35 vs. 6/35，p < 0.001）。两种TIC在组织学分级、MVD、VEGF、Ki 67表达及血清CA 125水平上均有显著性差异（均P < 0.01）。Ktranss、Kep和IAUC值在总体和成对比较中显示出不同组织学分级中的显著差异，除了2级与3级中的IAUC（所有p < 0.01）。三个年级组间Ve值差异无统计学意义（p > 0.05）。Ktrans、Kep和IAUC值与MVD、VEGF和Ki 67表达呈正相关（均P < 0.01）。Ve与MVD、VEGF表达、Ki 67表达及CA 125水平均无显著相关性（均p > 0.05）。DCE-MRI的TIC类型和灌注参数可以在一定程度上反映肿瘤的分级、血管生成和细胞增殖情况，从而帮助制定治疗方案和预测预后。
To investigate dynamic contrast enhanced magnetic resonance imaging (DCE-MRI) for assessing histopathological and molecular biological features in induced rat epithelial ovarian carcinomas (EOCs). 7,12-dimethylbenz[A]anthracene (DMBA) was applied to induce EOCs in situ in 46 SD rats. Conventional MRI and DCE-MRI were performed to evaluate the morphology and perfusion features of the tumors, including the time-signal intensity curve (TIC), volume transfer constant (Ktrans), rate constant (Kep), extravascular extracellular space volume ratio (Ve) and initial area under the curve (IAUC). DCE-MRI parameters were correlated with histological grade, microvascular density (MVD), vascular endothelial growth factor (VEGF) and fraction of Ki67-positive cells and the serum level of cancer antigen 125 (CA125). Thirty-five of the 46 rats developed EOCs. DCE-MRI showed type III TIC more frequently than type II (29/35 vs. 6/35, p < 0.001) in EOCs. The two types of TIC of tumors had significant differences in the histological grade, MVD, expression of VEGF and Ki67, and the serum level of CA125 (all p < 0.01). Ktrans, Kep and IAUC values showed significant differences in different histological grades in overall and pairwise comparisons except for IAUC in grade 2 vs. grade 3 (all p < 0.01). There was no significant difference in Ve values among the three grade groups (p > 0.05). Ktrans, Kep and IAUC values were positively correlated with MVD, VEGF and Ki67 expression (all p < 0.01). Ve was not significantly correlated with MVD, VEGF expression, Ki67 expression and the CA125 level (all p > 0.05). TIC types and perfusion parameters of DCE-MRI can reflect tumor grade, angiogenesis and cell proliferation to some extent, thereby helping treatment planning and predicting prognosis.
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影响因子：
--
作者：
Morgado M;Sutton MN;Simmons M;Warren CR;Lu Z;Constantinou PE;Liu J;Francis LL;Conlan RS;Bast RC Jr;Carson DD
通讯作者：
Carson DD
DOI：
10.4261/1305-3825.dir.2537-08.1
发表时间：
2010-09
期刊：
Diagnostic and interventional radiology (Ankara, Turkey)
影响因子：
--
作者：
Türkbey B;Thomasson D;Pang Y;Bernardo M;Choyke PL
通讯作者：
Choyke PL
动态增强磁共振成像在复杂卵巢肿瘤表征中的价值
影响因子：
4
作者：
Li HM;Qiang JW;Ma FH;Zhao SH
通讯作者：
Zhao SH
影响因子：
--
作者：
Li, Xian;Hu, Jun-Li;Zhao, Bin
通讯作者：
Zhao, Bin
影响因子：
2.6
作者：
Ren, J.;Huan, Y.;Yang, Y.
通讯作者：
Yang, Y.