A composite transcriptional signature differentiates responses towards closely related herbicides in Arabidopsis thaliana and Brassica napus.
A composite transcriptional signature differentiates responses towards closely related herbicides in Arabidopsis thaliana and Brassica napus.
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DOI：
10.1007/s11103-009-9590-y
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发表时间：
2010-03
影响因子：
5.1
通讯作者：
Olszyk, David M.
中科院分区：
文献类型：
--
作者：
Das, Malay;Reichman, Jay R.;Haberer, Georg;Welzl, Gerhard;Aceituno, Felipe F.;Mader, Michael T.;Watrud, Lidia S.;Pfleeger, Thomas G.;Gutierrez, Rodrigo A.;Schaeffner, Anton R.;Olszyk, David M.
关键词：
本研究利用Affymetrix ATH1基因阵列对拟南芥（Arabidopsis thaliana）对5种除草剂的特异性反应进行了分析，这5种除草剂含有针对氨基酸生物合成两个不同分支的活性成分。一种除草剂含有针对5-烯醇丙酮酰莽草酸-3-磷酸合成酶（EPSPS）的草甘膦，另外四种除草剂含有不同的抑制乙酰乳酸合成酶（ALS）的化合物。与含有草甘膦的除草剂只影响少数转录本不同，抑制ALS的除草剂通过与核糖体生物发生和翻译、次生代谢、细胞壁修饰和生长相关的转录改变揭示了许多作用。101个基因的表达模式提供了与其他主要胁迫反应不同的特异性复合特征，并在针对同一酶（ALS）或含有相同化学类别的活性成分（磺酰脲）的除草剂中有所区别。在甘蓝型油菜中鉴定出一组表达模式相似的同源基因，并能正确区分5种除草剂的暴露。我们的研究结果表明，在拟南芥和甘蓝型油菜中，有限数量的基因能够分类和区分对密切相关除草剂的反应，以及一个复杂的转录特征在物种间的可转移性。本文的在线版本（doi:10.1007/s11103-009-9590-y）包含补充资料，仅供授权用户使用。
In this study, genome-wide expression profiling based on Affymetrix ATH1 arrays was used to identify discriminating responses of Arabidopsis thaliana to five herbicides, which contain active ingredients targeting two different branches of amino acid biosynthesis. One herbicide contained glyphosate, which targets 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), while the other four herbicides contain different acetolactate synthase (ALS) inhibiting compounds. In contrast to the herbicide containing glyphosate, which affected only a few transcripts, many effects of the ALS inhibiting herbicides were revealed based on transcriptional changes related to ribosome biogenesis and translation, secondary metabolism, cell wall modification and growth. The expression pattern of a set of 101 genes provided a specific, composite signature that was distinct from other major stress responses and differentiated among herbicides targeting the same enzyme (ALS) or containing the same chemical class of active ingredient (sulfonylurea). A set of homologous genes could be identified in Brassica napus that exhibited a similar expression pattern and correctly distinguished exposure to the five herbicides. Our results show the ability of a limited number of genes to classify and differentiate responses to closely related herbicides in A. thaliana and B. napus and the transferability of a complex transcriptional signature across species. The online version of this article (doi:10.1007/s11103-009-9590-y) contains supplementary material, which is available to authorized users.
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影响因子：
5.1
作者：
Glombitza, S;Dubuis, PH;Schäffner, AR
通讯作者：
Schäffner, AR
影响因子：
6.1
作者：
Kelley, KB;Lambert, KN;Riechers, DE
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Riechers, DE
DOI：
10.1006/bbrc.2001.5350
发表时间：
2001-08-10
影响因子：
3.1
作者：
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Ott, KH
影响因子：
64.8
作者：
Hirsh, AE;Fraser, HB
通讯作者：
Fraser, HB
DOI：
10.1073/pnas.0706421104
发表时间：
2007-12-18
影响因子：
11.1
作者：
Brazier-Hicks, Melissa;Offen, Wendy A.;Edwards, Robert
通讯作者：
Edwards, Robert