Evidence for a fourteenth mtDNA-encoded protein in the female-transmitted mtDNA of marine Mussels (Bivalvia: Mytilidae).
Evidence for a fourteenth mtDNA-encoded protein in the female-transmitted mtDNA of marine Mussels (Bivalvia: Mytilidae).
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DOI：
10.1371/journal.pone.0019365
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发表时间：
2011-04-27
期刊：
影响因子：
3.7
通讯作者：
Hoeh WR
中科院分区：
文献类型：
--
作者：
Breton S;Ghiselli F;Passamonti M;Milani L;Stewart DT;Hoeh WR
最近已经建立了动物线粒体基因组的一个新特征：即，存在额外的、谱系特异性的、mtDNA编码的具有功能意义的蛋白质。这一特征已在线粒体DNA双单亲遗传（DUI）的淡水贻贝中观察到。后一种独特的mtDNA传递系统也存在于一些海洋贻贝和海洋蛤中，其特征在于一个mt基因组遗传自雌性亲本（F mtDNA）和一个mt基因组遗传自雄性亲本（M mtDNA）。在淡水贻贝中，新的mtDNA编码蛋白质已被证明是mt基因组特异性的（即，F基因组的一种新蛋白和M基因组的一种新蛋白）。有人推测，这些新的，F-和M-特异性，mtDNA编码的蛋白质（和/或其他F-和/或M-特异性mtDNA序列）可能是负责不同模式的mtDNA传输在双壳类，但这仍然有待证明。我们调查了所有完整的（或接近完整的）女性和男性传播的海洋贻贝mtDNA先前测序的ORF的存在下，可能有功能的重要性，在这些双壳类。我们的研究结果证实了一个新的F基因组特异性mt ORF的存在，显着的长度（> 100 aa），并位于控制区，最有可能在海洋贻贝的功能意义。在5个贻贝物种中鉴定出这个ORF表明它已经在贻贝科（贻贝亚科）中维持了1.13亿年。此外，该ORF可能在Musculista senhousia的F mt基因组中具有同源物，Musculista senhousia是Crenellinae亚科中含有DUI的mytild物种。我们目前的证据支持功能的F-特异性ORF在转录，氨基酸和核苷酸水平。我们的研究结果提供了支持的假设，即“新的F基因组特异性线粒体基因”参与的关键生物学功能的双壳类与DUI。
A novel feature for animal mitochondrial genomes has been recently established: i.e., the presence of additional, lineage-specific, mtDNA-encoded proteins with functional significance. This feature has been observed in freshwater mussels with doubly uniparental inheritance of mtDNA (DUI). The latter unique system of mtDNA transmission, which also exists in some marine mussels and marine clams, is characterized by one mt genome inherited from the female parent (F mtDNA) and one mt genome inherited from the male parent (M mtDNA). In freshwater mussels, the novel mtDNA-encoded proteins have been shown to be mt genome-specific (i.e., one novel protein for F genomes and one novel protein for M genomes). It has been hypothesized that these novel, F- and M-specific, mtDNA-encoded proteins (and/or other F- and/or M-specific mtDNA sequences) could be responsible for the different modes of mtDNA transmission in bivalves but this remains to be demonstrated. We investigated all complete (or nearly complete) female- and male-transmitted marine mussel mtDNAs previously sequenced for the presence of ORFs that could have functional importance in these bivalves. Our results confirm the presence of a novel F genome-specific mt ORF, of significant length (>100aa) and located in the control region, that most likely has functional significance in marine mussels. The identification of this ORF in five Mytilus species suggests that it has been maintained in the mytilid lineage (subfamily Mytilinae) for ∼13 million years. Furthermore, this ORF likely has a homologue in the F mt genome of Musculista senhousia, a DUI-containing mytilid species in the subfamily Crenellinae. We present evidence supporting the functionality of this F-specific ORF at the transcriptional, amino acid and nucleotide levels. Our results offer support for the hypothesis that “novel F genome-specific mitochondrial genes” are involved in key biological functions in bivalve species with DUI.
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