Coherent nonlinear optical imaging: beyond fluorescence microscopy.
Coherent nonlinear optical imaging: beyond fluorescence microscopy.
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DOI：
10.1146/annurev.physchem.012809.103512
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发表时间：
2011
影响因子：
14.7
通讯作者：
Xie XS
中科院分区：
文献类型：
--
作者：
Min W;Freudiger CW;Lu S;Xie XS
对具有光谱对比度而不是荧光的荧光检测灵敏度的追求导致了生物系统的光学显微镜的各种新方法。相干非线性光学成像，特别是最近发展起来的非线性耗散显微术（包括受激拉曼散射和双光子吸收）和泵浦探测显微术（包括受激发射、激发态吸收和基态耗尽），为非荧光物质提供了清晰而强大的图像对比度。由于采用高频调制传输方案，它们表现出卓越的检测灵敏度。通过直接询问分子的振动和/或电子能级，它们提供高分子特异性。在这里，我们审查的基本原则，激发和检测方案，以及示范性的生物医学应用这一类新兴的分子成像技术。
The quest for ultrahigh detection sensitivity with spectroscopic contrasts other than fluorescence has led to various novel approaches to optical microscopy of biological systems. Coherent nonlinear optical imaging, especially the recently developed nonlinear dissipation microscopy, including stimulated Raman scattering and two photon absorption, and pump-probe microscopy, including stimulated emission, excited state absorption and ground state depletion, provide distinct and powerful image contrasts for non-fluorescent species. Thanks to high-frequency modulation transfer scheme, they exhibit superb detection sensitivity. By directly interrogating vibrational and/or electronic energy levels of molecules, they offer high molecular specificity. Here we review the underlying principles, excitation and detection schemes, as well as exemplary biomedical applications of this emerging class of molecular imaging techniques.
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影响因子：
4
作者：
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通讯作者：
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56.9
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