Trophic Ecology of the Tropical Pacific Sponge Mycale grandis Inferred from Amino Acid Compound-Specific Isotopic Analyses
Trophic Ecology of the Tropical Pacific Sponge Mycale grandis Inferred from Amino Acid Compound-Specific Isotopic Analyses
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从氨基酸化合物特异性同位素分析推断热带太平洋海绵大菌的营养生态
DOI：
10.1007/s00248-019-01410-x
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发表时间：
2020
影响因子：
3.6
通讯作者：
Popp, Brian N.
中科院分区：
文献类型：
--
作者：
Shih, Joy L.;Selph, Karen E.;Wall, Christopher B.;Wallsgrove, Natalie J.;Lesser, Michael P.;Popp, Brian N.
许多海绵都有丰富和活跃的微生物群落，这些微生物群落可能在海绵全息吸收溶解有机物(DOM)的过程中发挥作用，尽管DOM吸收和代谢的机制尚不清楚。对热带太平洋浅水海绵Mycale Grandissis的整体海绵、分离的海绵细胞和分离的共生微生物细胞进行了整体和化合物特定同位素分析，以阐明宿主海绵与其伴生微生物群落之间的营养关系。巨型分枝杆菌海绵细胞中氨基酸的δ15N和δ13C值与其细菌共生体中氨基酸的15N和13C值无差异。因此，海绵及其共生微生物的营养位置没有差异，表明颗粒状海绵细胞分离株不表现出典型的后生动物摄食的氨基酸同位素特征。此外，分离的微生物组分和海绵细胞组分都具有类似高的ΣV值--一种衡量细菌重新合成有机物的指标，由营养氨基酸的各个δ15N值之间的方差和计算得出。在海绵中观察到的这些高ΣV值表明，大微藻不依赖于光共生体转移的光合作用产物或摄食水柱微浮游动物，而是通过摄取细菌来源的氨基酸来获得营养。我们的结果表明，从共生体中直接同化细菌合成的氨基酸，无论是以类似于在珊瑚全硅藻中观察到的移位的方式，还是通过吞噬摄食，都是巨藻获取氨基酸的一个重要的(如果不是主要的)途径。
Many sponges host abundant and active microbial communities that may play a role in the uptake of dissolved organic matter (DOM) by the sponge holobiont, although the mechanism of DOM uptake and metabolism is uncertain. Bulk and compound-specific isotopic analysis of whole sponge, isolated sponge cells, and isolated symbiotic microbial cells of the shallow water tropical Pacific spongeMycale grandiswere used to elucidate the trophic relationships between the host sponge and its associated microbial community. δ15N and δ13C values of amino acids inM.grandisisolated sponge cells are not different from those of its bacterial symbionts. Consequently, there is no difference in trophic position of the sponge and its symbiotic microbes indicating thatM.grandissponge cell isolates do not display amino acid isotopic characteristics typical of metazoan feeding. Furthermore, both the isolated microbial and sponge cell fractions were characterized by a similarly high ΣV value—a measure of bacterial-re-synthesis of organic matter calculated from the sum of variance among individual δ15N values of trophic amino acids. These high ΣV values observed in the sponge suggest thatM.grandisis not reliant on translocated photosynthate from photosymbionts or feeding on water column picoplankton, but obtains nutrition through the uptake of amino acids of bacterial origin. Our results suggest that direct assimilation of bacterially synthesized amino acids from its symbionts, either in a manner similar to translocation observed in the coral holobiont or through phagotrophic feeding, is an important if not primary pathway of amino acid acquisition forM.grandis.
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DOI：
--
发表时间：
2009
期刊：
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影响因子：
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作者：
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10.1016/s1936-7961(07)01012-3
发表时间：
2007
期刊：
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影响因子：
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