3D Printing of Microgel Scaffolds with Tunable Void Fraction to Promote Cell Infiltration.
3D Printing of Microgel Scaffolds with Tunable Void Fraction to Promote Cell Infiltration.
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DOI：
10.1002/adhm.202100644
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发表时间：
2021-09
影响因子：
10
通讯作者：
Heilshorn SC
中科院分区：
文献类型：
--
作者：
Seymour AJ;Shin S;Heilshorn SC
颗粒状、微凝胶基材料由于其固有的多孔性而作为有前途的组织工程支架引起了人们的兴趣，这可以促进细胞浸润。使这些材料适应3D生物打印，同时保持足够的空隙空间以使细胞迁移，可能是具有挑战性的，因为决定可打印性的流变特性受到微凝胶填充和空隙率的强烈影响。在这项工作中，我们提出了一种策略，以解耦印刷适性和空隙率共混紫外线交联明胶甲基丙烯酰（GelMA）微凝胶与牺牲明胶微凝胶形成复合油墨。我们观察到表观粘度大于约100 Pa·s（对应于微凝胶浓度≥ 5重量%）的油墨具有流变特性，能够在空气中基于挤出印刷多层结构。通过改变GelMA与牺牲明胶微凝胶的比率，同时保持总浓度恒定在6wt%，我们创建了一系列GelMA：明胶微凝胶油墨，其允许将空隙率从0.20调整到0.57。此外，观察到接种到打印的构建体上的人脐静脉内皮细胞（HUVEC）以空隙率依赖性方式迁移到颗粒状油墨中。因此，我们的微凝胶墨水系列有望用于依赖细胞浸润的3D打印和组织工程应用。基于微凝胶的材料的固有多孔性使它们有希望促进细胞浸润。但是，使这些材料适应3D生物打印需要使用强烈影响孔隙率的方法来优化流变特性。报道了一种通过共混UV可交联微凝胶和牺牲微凝胶来解耦空隙空间和可印刷性的方法。所得的油墨族具有影响细胞渗透的空隙率。
Granular, microgel-based materials have garnered interest as promising tissue engineering scaffolds due to their inherent porosity, which can promote cell infiltration. Adapting these materials for 3D bioprinting, while maintaining sufficient void space to enable cell migration, can be challenging, since the rheological properties that determine printability are strongly influenced by microgel packing and void fraction. In this work, we propose a strategy to decouple printability and void fraction by blending UV-crosslinkable gelatin methacryloyl (GelMA) microgels with sacrificial gelatin microgels to form composite inks. We observe that inks with an apparent viscosity greater than ~100 Pa⋅s (corresponding to microgel concentrations ≥ 5 wt%) have rheological properties that enable extrusion-based printing of multilayered structures in air. By altering the ratio of GelMA to sacrificial gelatin microgels, while holding total concentration constant at 6 wt%, we create a family of GelMA:gelatin microgel inks that allows for tuning of void fraction from 0.20 to 0.57. Furthermore, human umbilical vein endothelial cells (HUVEC) seeded onto printed constructs are observed to migrate into granular inks in a void fraction-dependent manner. Thus, our family of microgel inks holds promise for use in 3D printing and tissue engineering applications that rely upon cell infiltration. The inherent porosity of microgel-based materials makes them promising for promoting cell infiltration. But adapting these materials to 3D bioprinting requires optimizing rheological properties using methods that strongly influence porosity. A method is reported to decouple void space and printability by blending UV-crosslinkable and sacrificial microgels. The resulting family of inks have void fractions that influence cellular infiltration.
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DOI：
10.3390/ma11030454
发表时间：
2018-03-20
期刊：
Materials (Basel, Switzerland)
影响因子：
--
作者：
Habib A;Sathish V;Mallik S;Khoda B
通讯作者：
Khoda B
DOI：
10.1002/advs.202001419
发表时间：
2020-09
期刊：
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
影响因子：
--
作者：
Kessel B;Lee M;Bonato A;Tinguely Y;Tosoratti E;Zenobi-Wong M
通讯作者：
Zenobi-Wong M
影响因子：
10
作者：
Darling, Nicole J.;Xi, Weixian;Segura, Tatiana
通讯作者：
Segura, Tatiana
影响因子：
13.6
作者：
Freeman, Fiona E.;Pitacco, Pierluca;Kelly, Daniel J.
通讯作者：
Kelly, Daniel J.
影响因子：
--
作者：
Griffith, CK;Miller, C;George, SC
通讯作者：
George, SC