3D Bioprinting of Macroporous Materials Based on Entangled Hydrogel Microstrands.
3D Bioprinting of Macroporous Materials Based on Entangled Hydrogel Microstrands.
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DOI：
10.1002/advs.202001419
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发表时间：
2020-09
期刊：
影响因子：
--
通讯作者：
Zenobi-Wong M
中科院分区：
文献类型：
--
作者：
Kessel B;Lee M;Bonato A;Tinguely Y;Tosoratti E;Zenobi-Wong M
水凝胶是哺乳动物细胞外基质的极好模拟物，在组织工程中有广泛的应用。然而，整体水凝胶的纳米多孔性限制了关键生物分子的质量传输。3D生物打印中使用的微凝胶既可以实现定制形状，也可以大大提高对一系列细胞功能的允许度，但是基于球形微珠的生物墨水具有挑战性，具有固有的各向同性，并且需要二次交联。在这里，基于高纵横比水凝胶微链的生物墨水被引入来克服这些限制。预交联的散装水凝胶通过孔径为40-100微米的网格被分解成微链。微链是可塑的，形成一个多孔的，纠缠的结构，在水介质中稳定，没有进一步的交联。缠结微链具有良好的生物墨水流变性能，可用于挤出生物打印。此外，单个微链在挤压过程中排列，并促进肌管的排列。细胞可以放置在水凝胶相的内部或外部，存活率为90%。与微链共同打印的软骨细胞沉积了丰富的细胞外基质，培养6周后，模量从2.7增加到780.2 kPa。这种将大块水凝胶分解成先进生物墨水的强大方法既可扩展又通用，代表了结构水凝胶3D生物打印的重要工具箱。散装凝胶被分解成水凝胶微链，形成具有组织工程有趣特性的结构材料。这种大孔材料的制备简单，对细胞友好，并且与材料无关。单个微链通过挤压排列，促进生物结构的定向。缠绕微链是一种很好的挤出生物3D打印的生物链，可以作为组织工程的平台。
Hydrogels are excellent mimetics of mammalian extracellular matrices and have found widespread use in tissue engineering. Nanoporosity of monolithic bulk hydrogels, however, limits mass transport of key biomolecules. Microgels used in 3D bioprinting achieve both custom shape and vastly improved permissivity to an array of cell functions, however spherical‐microbead‐based bioinks are challenging to upscale, are inherently isotropic, and require secondary crosslinking. Here, bioinks based on high‐aspect‐ratio hydrogel microstrands are introduced to overcome these limitations. Pre‐crosslinked, bulk hydrogels are deconstructed into microstrands by sizing through a grid with apertures of 40–100 µm. The microstrands are moldable and form a porous, entangled structure, stable in aqueous medium without further crosslinking. Entangled microstrands have rheological properties characteristic of excellent bioinks for extrusion bioprinting. Furthermore, individual microstrands align during extrusion and facilitate the alignment of myotubes. Cells can be placed either inside or outside the hydrogel phase with >90% viability. Chondrocytes co‐printed with the microstrands deposit abundant extracellular matrix, resulting in a modulus increase from 2.7 to 780.2 kPa after 6 weeks of culture. This powerful approach to deconstruct bulk hydrogels into advanced bioinks is both scalable and versatile, representing an important toolbox for 3D bioprinting of architected hydrogels. Bulk gels are deconstructed into hydrogel microstrands to form structured materials with interesting properties for tissue engineering. Preparation of this macroporous material is facile, cell‐friendly, and material independent. Individual microstrands align through extrusion and facilitate orientation in biological structures. Entangled microstrands are an excellent bioink for extrusion 3D bioprinting and can be utilized as a platform for tissue engineering.
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DOI：
10.3390/ma11071116
发表时间：
2018-06-29
期刊：
Materials (Basel, Switzerland)
影响因子：
--
作者：
Beldjilali-Labro M;Garcia Garcia A;Farhat F;Bedoui F;Grosset JF;Dufresne M;Legallais C
通讯作者：
Legallais C
DOI：
10.3390/ma11030454
发表时间：
2018-03-20
期刊：
Materials (Basel, Switzerland)
影响因子：
--
作者：
Habib A;Sathish V;Mallik S;Khoda B
通讯作者：
Khoda B
用于三维细胞微环境工程的功能和仿生材料
影响因子：
62.1
作者：
Huang G;Li F;Zhao X;Ma Y;Li Y;Lin M;Jin G;Lu TJ;Genin GM;Xu F
通讯作者：
Xu F
DOI：
10.1073/pnas.0811484106
发表时间：
2009-04-14
影响因子：
11.1
作者：
McWhirter, J. Liam;Noguchi, Hiroshi;Gompper, Gerhard
通讯作者：
Gompper, Gerhard
影响因子：
5.7
作者：
Costantini M;Testa S;Fornetti E;Barbetta A;Trombetta M;Cannata SM;Gargioli C;Rainer A
通讯作者：
Rainer A