3D Printability of Alginate-Carboxymethyl Cellulose Hydrogel.
3D Printability of Alginate-Carboxymethyl Cellulose Hydrogel.
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DOI：
10.3390/ma11030454
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发表时间：
2018-03-20
期刊：
影响因子：
--
通讯作者：
Khoda B
中科院分区：
文献类型：
--
作者：
Habib A;Sathish V;Mallik S;Khoda B
三维（3D）生物打印是一种革命性的技术，通过控制生物材料的逐层沉积沿着细胞的高精度定位，在体外复制3D功能活组织支架。由于其生物相容性，天然水凝胶通常被认为是支架材料。然而，水凝胶材料的机械完整性，特别是在3D支架结构中，是一个问题。在这项研究中，一种新的杂化水凝胶，即海藻酸钠与羧甲基纤维素（CMC）的开发和系统的定量表征测试进行验证，其印刷性，形状保真度和细胞活力。流变性能测试、力学性能测试、单丝收缩和熔融性能测试结果表明，纤维具有良好的形状保真度。用胰腺癌细胞BxPC 3制造三维支架结构，并在23天后记录86%的细胞活力。这种混合水凝胶可以是3D生物打印过程中的潜在生物材料，并且概述的表征技术开辟了一条指导可再现的可打印性和形状保真度的途径。
Three-dimensional (3D) bio-printing is a revolutionary technology to reproduce a 3D functional living tissue scaffold in-vitro through controlled layer-by-layer deposition of biomaterials along with high precision positioning of cells. Due to its bio-compatibility, natural hydrogels are commonly considered as the scaffold material. However, the mechanical integrity of a hydrogel material, especially in 3D scaffold architecture, is an issue. In this research, a novel hybrid hydrogel, that is, sodium alginate with carboxymethyl cellulose (CMC) is developed and systematic quantitative characterization tests are conducted to validate its printability, shape fidelity and cell viability. The outcome of the rheological and mechanical test, filament collapse and fusion test demonstrate the favorable shape fidelity. Three-dimensional scaffold structures are fabricated with the pancreatic cancer cell, BxPC3 and the 86% cell viability is recorded after 23 days. This hybrid hydrogel can be a potential biomaterial in 3D bioprinting process and the outlined characterization techniques open an avenue directing reproducible printability and shape fidelity.
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影响因子：
--
作者：
Blaeser A;Duarte Campos DF;Weber M;Neuss S;Theek B;Fischer H;Jahnen-Dechent W
通讯作者：
Jahnen-Dechent W
影响因子：
46.9
作者：
Kang, Hyun-Wook;Lee, Sang Jin;Atala, Anthony
通讯作者：
Atala, Anthony
影响因子：
9
作者：
Iwami, K.;Noda, T.;Umeda, N.
通讯作者：
Umeda, N.
研究3D生物打印中水凝胶的可打印性。
影响因子：
4.6
作者：
He Y;Yang F;Zhao H;Gao Q;Xia B;Fu J
通讯作者：
Fu J
影响因子：
4.1
作者：
Han, Yingying;Wang, Lijuan
通讯作者：
Wang, Lijuan