A highly sensitive, quick and simple quantification method for nicotianamine and 2'-deoxymugineic acid from minimum samples using LC/ESI-TOF-MS achieves functional analysis of these components in plants.
A highly sensitive, quick and simple quantification method for nicotianamine and 2'-deoxymugineic acid from minimum samples using LC/ESI-TOF-MS achieves functional analysis of these components in plants.
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DOI：
10.1093/pcp/pcp141
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发表时间：
2009-11
影响因子：
4.9
通讯作者：
Nishizawa NK
中科院分区：
文献类型：
--
作者：
Kakei Y;Yamaguchi I;Kobayashi T;Takahashi M;Nakanishi H;Yamakawa T;Nishizawa NK
建立了液相色谱/电喷雾电离飞行时间质谱（LC/ESI-TOF-MS）分析烟碱胺（NA）和2′-脱氧mugineacid （DMA）的高灵敏度定量方法。用9-氟酰甲氧羰基氯衍生NA和DMA的氨基和羟基。对富铁和缺铁条件下栽培的水稻10 μl木质部汁液中NA和DMA的含量进行了定量测定。在富铁植物中，NA和DMA的浓度与Fe的浓度基本相等。缺铁植株NA浓度变化不显著，而DMA浓度显著升高。
A highly sensitive quantitative method for assaying nicotianamine (NA) and 2′-deoxymugineic acid (DMA) using liquid chromatography/electrospray ionization time-of-flight mass spectrometry (LC/ESI-TOF-MS) was developed. The amino and hydroxyl groups of NA and DMA were derivatized using 9-fluorenylmethoxycarbonyl chloride. The amounts of NA and DMA in 10 μl of xylem sap from rice cultivated under iron (Fe)-sufficient and Fe-deficient conditions were quantified without concentration. In Fe-sufficient plants, the concentrations of NA and DMA were almost equal to that of Fe. In Fe-deficient plants, the concentration of NA did not change significantly, whereas that of DMA increased markedly.
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影响因子：
2.9
作者：
MIYANO, H;TOYOOKA, T;NAKAJIMA, T
通讯作者：
NAKAJIMA, T
影响因子：
7.4
作者：
von Wirén, N;Klair, S;Hider, RC
通讯作者：
Hider, RC
影响因子：
2.9
作者：
Xuan, Yue;Scheuermann, Enrico B.;Weber, Gunther
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Weber, Gunther
影响因子：
7.4
作者：
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通讯作者：
Abadía, J
影响因子：
4.3
作者：
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通讯作者：
Römheld, V