Adipose stromal/stem cells assist fat transplantation reducing necrosis and increasing graft performance.
Adipose stromal/stem cells assist fat transplantation reducing necrosis and increasing graft performance.
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DOI：
10.1007/s10495-013-0878-7
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发表时间：
2013-10
期刊：
影响因子：
7.2
通讯作者：
Dominici, Massimo
中科院分区：
文献类型：
--
作者：
Piccinno, Maria Serena;Veronesi, Elena;Loschi, Pietro;Pignatti, Marco;Murgia, Alba;Grisendi, Giulia;Castelli, Ilaria;Bernabei, Daniela;Candini, Olivia;Conte, Pierfranco;Paolucci, Paolo;Horwitz, Edwin M.;De Santis, Giorgio;Iughetti, Lorenzo;Dominici, Massimo
自体脂肪移植(AFT)是一种用于修复创伤、烧伤、肿瘤切除和脂肪营养不良后的脂肪组织(AT)的手术，但由于移植物缺乏持久性而受到负面影响。这一不良结果背后的原因尚不清楚，似乎涉及收获/移植的成熟脂肪细胞或其间充质前体细胞(即脂肪基质/干细胞(ASC))的损害，以及移植后AT的凋亡和退化。本研究首先建立了兔皮下血管再生模型，以评估植入后不同时间的移植物质量，重点观察相关参数，如坏死和血管生成。将标准AFT与纯化的自体ASC结合透明质酸(HA)辅助AFT的策略进行比较。500万自体体外分离的CD29+、CD90+、CD49e+ASC负载到HA中，浓缩1mlAT，在保留移植AT构筑的同时，对减少AFT坏死和增加血管生成具有早期显著的保护作用。ASC辅助AFT的这一有益影响随后在三个月后得到证实，脂肪保存良好，没有细胞转化的迹象。通过一种新的ASC辅助AFT方法，我们确保了早期细胞死亡的减少，有利于持久的移植物性能，可能对患者有更稳定的益处。本文的在线版本(doi：10.1007/s10495-0130878-7)包含补充材料，授权用户可以使用。
Autologous fat transfer (AFT) is a procedure for adipose tissue (AT) repair after trauma, burns, post-tumor resections and lipodystrophies still negatively impacted by the lack of graft persistence. The reasons behind this poor outcome are unclear and seem to involve damages in either harvested/transplanted mature adipocytes or on their mesenchymal progenitors, namely adipose stromal/stem cells (ASC), and due to post-transplant AT apoptosis and involution. A rabbit subcutaneous AT regeneration model was here developed to first evaluate graft quality at different times after implant focusing on related parameters, such as necrosis and vasculogenesis. Standard AFT was compared with a strategy where purified autologous ASC, combined with hyaluronic acid (HA), assisted AFT. Five million of autologous ex vivo isolated CD29+, CD90+, CD49e+ ASC, loaded into HA, enriched 1 ml of AT generating an early significant protective effect in reducing AFT necrosis and increasing vasculogenesis with a preservation of transplanted AT architecture. This beneficial impact of ASC assisted AFT was then confirmed at three months with a robust lipopreservation and no signs of cellular transformation. By a novel ASC assisted AFT approach we ensure a reduction in early cell death favoring an enduring graft performance possibly for a more stable benefit in patients. The online version of this article (doi:10.1007/s10495-013-0878-7) contains supplementary material, which is available to authorized users.
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影响因子：
3.2
作者：
Lin, WQ;Shuster, S;Stern, R
通讯作者：
Stern, R
脂肪来源的间充质干细胞通过 PI3-K/Akt 激活上调 XIAP，保护 PC12 细胞免受谷氨酸兴奋性毒性诱导的细胞凋亡。
影响因子：
4.5
作者：
Lu, Shan;Lu, Chunhua;Zhao, Robert Chunhua
通讯作者：
Zhao, Robert Chunhua
影响因子：
4.1
作者：
Casteilla, Louis;Planat-Benard, Valerie;Cousin, Beatrice
通讯作者：
Cousin, Beatrice
影响因子：
2.8
作者：
de Girolamo, L.;Arrigoni, E.;Brini, A. T.
通讯作者：
Brini, A. T.
影响因子：
2.7
作者：
Chen, L.;Peng, E. J.;Ye, Z. Q.
通讯作者：
Ye, Z. Q.