Effect of melatonin on the proliferation and differentiation of chondrocytes from rat vertebral body growth plate in vitro.
Effect of melatonin on the proliferation and differentiation of chondrocytes from rat vertebral body growth plate in vitro.
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DOI：
10.7150/ijms.5645
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发表时间：
2013
影响因子：
3.6
通讯作者：
Chen JT
中科院分区：
文献类型：
--
作者：
Zhong ZM;Li T;Xu ZX;Meng TT;Zeng JH;Zheng S;Ye WB;Wu Q;Chen JT
目的：椎体生长板（VBGP）生长异常被认为是青少年特发性脊柱侧凸（AIS）的病因之一。众所周知，褪黑素与AIS的发生发展相关。本研究旨在探讨褪黑素对体外培养大鼠VBGP软骨细胞的影响。方法：从大鼠VBGP中分离软骨细胞，给予或不给予褪黑素处理。用Alamar Blue法测定细胞增殖。实时荧光定量PCR检测ⅱ型胶原蛋白和聚集蛋白的基因表达。Western blotting检测褪黑激素受体（MT1, MT2），增殖细胞核抗原（PCNA，细胞增殖标志物），Sox9（软骨细胞分化标志物）和Smad4（调节软骨细胞增殖和分化的常见介质）的表达。结果：大鼠VBGP软骨细胞中检测到褪黑素受体MT1、MT2的表达。褪黑素在10和100µg/mL浓度下，显著抑制vbgp -软骨细胞的增殖和II型胶原和聚集蛋白的基因表达，下调PCNA、Sox9和Smad4的蛋白表达。此外，褪黑激素的抑制作用被一种褪黑激素受体拮抗剂——卢津多所逆转。结论：上述结果提示高浓度褪黑素可抑制VBGP软骨细胞的增殖和分化，这可能为AIS的发病机制提供新的认识。
Purpose: Abnormal growth of vertebral body growth plate (VBGP) is considered as one of the etiologic factors in the adolescent idiopathic scoliosis (AIS). It was well-known that melatonin was correlated with the emergence and development of AIS. This study aimed to investigate the effect of melatonin on rat VBGP chondrocytes in vitro. Methods:Chondrocytes were isolated from rat VBGP, and treated with or without melatonin. Cell proliferation was measured by the Alamar Blue assay. Gene expression of collagen type II and aggrecan were evaluated by real-time PCR. Expression of the melatonin receptors (MT1, MT2), proliferating cell nuclear antigen (PCNA, a cell proliferation marker), Sox9 (a chondrocytic differentiation marker) and Smad4 (a common mediator in regulating the proliferation and differentiation of chondrocytes) were detected by Western blotting. Results: Expression of melatonin receptors (MT1, MT2) were detected in the rat VBGP chondrocytes. Melatonin, at 10 and 100 µg/mL concentration, significantly inhibited the proliferation of VBGP-chondrocytes and the gene expression of collagen type II and aggrecan, and down-regulated the protein expression of PCNA, Sox9 and Smad4. In addition, the inhibitory effect of melatonin was reversed by luzindole, a melatonin receptor antagonist. Conclusions: These results suggest that melatonin at high concentrations can inhibit the proliferation and differentiation of VBGP chondrocytes, which might give some new insight into the pathogenic mechanism of AIS.
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影响因子：
10.5
作者：
Akiyama, H;Chaboissier, MC;de Crombrugghe, B
通讯作者：
de Crombrugghe, B
影响因子：
10.3
作者：
Machida, Masafumi;Dubousset, Jean;Kimura, Jun
通讯作者：
Kimura, Jun
影响因子：
--
作者：
MANNING, WK;BONNER, WM
通讯作者：
BONNER, WM
影响因子：
2.8
作者：
BREUR, GJ;VANENKEVORT, BA;WILSMAN, NJ
通讯作者：
WILSMAN, NJ
影响因子：
4.2
作者：
Azeddine, Bouziane;Letellier, Kareen;Moreau, Alain
通讯作者：
Moreau, Alain