Effects of different solid carbon sources on water quality, biofloc quality and gut microbiota of Nile tilapia (Oreochromis niloticus) larvae
Effects of different solid carbon sources on water quality, biofloc quality and gut microbiota of Nile tilapia (Oreochromis niloticus) larvae
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不同固体碳源对尼罗罗非鱼（Oreochromis niloticus）幼鱼水质、生物絮团质量和肠道菌群的影响
DOI：
10.1016/j.aquaculture.2018.06.078
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发表时间：
2018-10
期刊：
影响因子：
4.5
通讯作者：
Liu Dezhao
中科院分区：
文献类型：
--
作者：
Li Jiawei;Liu Gang;Li Changwei;Deng Yale;Tadda Musa Abubakar;Lan Lihua;Zhu Songming;Liu Dezhao
生物絮体系统需要外部碳源来维持细菌生物量生长的最佳C/N比。采用龙眼粉、聚羟基丁酸-羟基戊酸酯/龙眼粉（PHBVL）和聚丁二酸丁二醇酯/龙眼粉（PBSL）3种固相可生物降解复合物，对9个尼罗罗非鱼（Oreochromis niloticus）幼鱼养殖池进行了3次投喂试验。在原位生物絮体系统中应用LP作为“对照组”（3个罐），而在非原位生物絮体系统中使用PHBVL和PBSL（6个罐）。在120天的实验中，LP、PHBVL和PBSL组的C/N比分别维持在24.87 ± 5.66、22.93 ± 3.20和23.12 ± 3.54。LP、PHBVL和PBSL组之间的平均总氨氮（TAN）浓度无显著差异（P> 0.05）（1.10 ± 1.18、0.67 ± 0.38和1.18 ± 1.40 mg L-1）。LP组、PHBVL组和PBSL组的平均NO2−-N浓度（0.26 ± 0.38、0.01 ± 0.01和0.08 ± 0.12 mg L−1）存在显著差异（P<0.05）。LP组NO3−-N的积累（>40 mg L− 1）显著高于PHBVL和PBSL组（约2-3 mg L− 1）（P< .05）。为了表征生物絮体的质量，测量了三种处理的中值直径（D50）和必需氨基酸指数（EAAI）。D50分别为124.7 ± 4.24、131.6 ± 2.83和175.5 ± 9.19 μm，EAAI分别为0.969 ± 0.011、1.007 ± 0.014和0.995 ± 0.012，表明LP、PHBVL和PBSL组的生物絮体质量较好，能够满足水产动物的饲养要求。高通量测序结果表明，固体碳源不仅对生物絮体中的微生物群落结构有显著影响，而且对鱼肠道菌群的组成也有显著影响，16 SrRNA测序结果显示，各处理中芽孢杆菌均占优势（LP、PHBVL和PBSL分别占48.34%、49.24%和50.47%）。总体而言，LP与可生物降解聚合物作为碳源的混合物显示出显着更高的去除效率的硝酸盐和亚硝酸盐氮，和更高的生物絮质量比使用LP作为唯一的碳源。不同固体碳源对鱼类生长性能和健康的影响有待进一步研究。
External carbon source is needed for biofloc system to maintain an optimal C/N ratio for the growth of bacteria biomass. In this study, three solid-phase biodegradable compounds, including Longan powder (LP), polyhydroxybutyrate-hydroxyvalerate/LP (PHBVL) and Poly(butylene succinate)/LP (PBSL), were utilized to feed biofloc-based aquaculture systems in triplicates for nine Nile tilapia (Oreochromis niloticus) larvae culture tanks. LP was applied in the in-situ biofloc system as a “control group” (3 tanks), while PHBVL and PBSL were used in the ex-situ biofloc systems (6 tanks). During the 120-days experiment, the C/N ratio was maintained at 24.87 ± 5.66, 22.93 ± 3.20 and 23.12 ± 3.54 for the LP, PHBVL and PBSL groups, respectively. There were no significant differences (P> .05) of the averaged total ammonia nitrogen (TAN) concentration among the LP, PHBVL and PBSL groups (1.10 ± 1.18, 0.67 ± 0.38 and 1.18 ± 1.40 mg L−1). Significant differences of the averaged NO2−-N concentrations (0.26 ± 0.38, 0.01 ± 0.01 and 0.08 ± 0.12 mg L−1) were detected among the LP, PHBVL and PBSL groups (P< .05). The accumulation of NO3−-N in LP group (>40 mg L−1on day 120) was significantly higher than that of PHBVL and PBSL groups (about 2–3 mg L−1on day 120) (P< .05). To characterize the quality of biofloc, the median diameters (D50) and essential amino acids index (EAAI) were measured for three treatments. The D50 (124.7 ± 4.24, 131.6 ± 2.83 and 175.5 ± 9.19 μm) and EAAI (0.969 ± 0.011, 1.007 ± 0.014 and 0.995 ± 0.012) showed that the high quality bioflocs in the LP, PHBVL and PBSL groups could meet the requirement for feeding the aquatic animals. In addition, high throughput sequencing test showed that solid carbon source not only had a significant effect on the microbial community in bioflocs, but also on the composition of fish gut microbiota.Bacilluswas the dominating genus discovered in all treatments (48.34% in LP, 49.24% in PHBVL and 50.47% in PBSL) by 16S rRNA sequencing. Overall, blending LP with biodegradable polymers as carbon source showed significantly higher removal efficiency of nitrate and nitrite nitrogen, and higher biofloc quality than using LP as the only carbon source. How exactly various solid carbon sources influence fish growth performance and health need further study.
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影响因子：
4.5
作者：
Guozhi Luo;N. Zhang;Shengli Cai;Hongxin Tan;Zefeng Liu
通讯作者：
Guozhi Luo;N. Zhang;Shengli Cai;Hongxin Tan;Zefeng Liu
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作者：
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通讯作者：
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影响因子：
0.4
作者：
S. Arai
通讯作者：
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影响因子：
4.5
作者：
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通讯作者：
Verdegem, MCJ