Probing the mechanisms of electron capture dissociation mass spectrometry with nitrated peptides.
Probing the mechanisms of electron capture dissociation mass spectrometry with nitrated peptides.
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探测电子与硝酸肽捕获分离质谱法的机理。
DOI：
10.1039/c0cp00623h
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发表时间：
2010-11-07
期刊：
影响因子：
--
通讯作者：
Cooper HJ
中科院分区：
文献类型：
--
作者：
Jones AW;Cooper HJ
利用硝化胜肽探讨电子捕获解离质谱之机制层级。以前，我们已经表明，3-硝基酪氨酸的肽序列内的存在下，严重耗尽的肽骨架片段通常观察到以下电子捕获解离（ECD）质谱。相反，硝化肽的ECD的特征在于大量损失小的中性物质（羟基自由基、水和氨）。在这里，我们调查氨损失的起源，通过比较的ECD行为的赖氨酸和精氨酸含有硝化肽，和它们的N-乙酰化的对应物，和硝化肽不含碱性氨基酸残基。结果表明，氨损失来自N-末端的肽，然而，这项工作的关键发现是提供到各种建议的ECD机制的层次结构的洞察力：Utah-Washington机制，电子捕食者机制和奥斯陆机制。
Investigation into the hierarchy of the mechanisms of electron capture dissociation mass spectrometry by use of nitrated peptides. Previously we have shown that the presence of 3-nitrotyrosine within a peptide sequence severely depletes the peptide backbone fragments typically observed following electron capture dissociation (ECD) mass spectrometry. Instead, ECD of nitrated peptides is characterised by abundant losses of small neutrals (hydroxyl radicals, water and ammonia). Here, we investigate the origin of ammonia loss by comparing the ECD behaviour of lysine- and arginine-containing nitrated peptides, and their N-acetylated counterparts, and nitrated peptides containing no basic amino acid residues. The results reveal that ammonia loss derives from the N-terminus of the peptides, however, the key finding of this work is the insight provided into the hierarchy of various proposed ECD mechanisms: the Utah-Washington mechanism, the electron predator mechanism and the Oslo mechanism.
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影响因子：
1.3
作者：
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通讯作者：
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