Mechano-chemical selections of two competitive unfolding pathways of a single DNA i-motif
Mechano-chemical selections of two competitive unfolding pathways of a single DNA i-motif
复制标题
单个 DNA i 基序的两条竞争性展开途径的机械化学选择
DOI：
10.1088/1674-1056/23/6/068702
复制
发表时间：
2014-06
影响因子：
1.7
通讯作者：
Yan Jie
中科院分区：
文献类型：
--
作者：
Xu Yue;Chen Hu;Qu Yu-Jie;Efremov, Artem K.;Li Ming;Ouyang Zhong-Can;Liu Dong-Sheng;Yan Jie
DNA i基序是在微酸性条件下在富含胞嘧啶的串联序列中形成的四链体结构。除了被认为是DNA纳米器件的构建模块之外，它还可能在调节染色体稳定性和基因转录方面发挥潜在的作用。i-motif的稳定性对这些功能至关重要。在这项工作中，我们研究了在人类端粒序列5′-（CCCTAA）3CCC中形成的单个i基序的机械稳定性，这揭示了一种新的pH和加载速率依赖的双峰去折叠力分布。虽然双峰解折叠力物种的原因尚不清楚，我们提出了一个现象学模型，涉及在较低的加载速率或较高的pH值有利于直接解折叠，这是受到竞争与另一个解折叠途径通过机械稳定的中间状态，其性质尚未确定。总之，i-motif-独特的机械化学响应为其稳定性提供了新的视角，这可能有助于指导设计新的基于i-motif-的DNA机械纳米器件。
The DNA i-motif is a quadruplex structure formed in tandem cytosine-rich sequences in slightly acidic conditions. Besides being considered as a building block of DNA nano-devices, it may also play potential roles in regulating chromosome stability and gene transcriptions. The stability of i-motif is crucial for these functions. In this work, we investigated the mechanical stability of a single i-motif formed in the human telomeric sequence 5′-(CCCTAA)3CCC, which revealed a novel pH and loading rate-dependent bimodal unfolding force distribution. Although the cause of the bimodal unfolding force species is not clear, we proposed a phenomenological model involving a direct unfolding favored at lower loading rate or higher pH value, which is subject to competition with another unfolding pathway through a mechanically stable intermediate state whose nature is yet to be determined. Overall, the unique mechano—chemical responses of i-motif-provide a new perspective to its stability, which may be useful to guide designing new i-motif-based DNA mechanical nano-devices.
登录
查看更多内容
影响因子：
14.9
作者：
Xu Y;Sugiyama H
通讯作者：
Sugiyama H
影响因子：
14.9
作者：
Guo K;Gokhale V;Hurley LH;Sun D
通讯作者：
Sun D
影响因子：
8.6
作者：
Yan, J;Marko, JF
通讯作者：
Marko, JF
影响因子：
3.1
作者：
Lagarias, JC;Reeds, JA;Wright, PE
通讯作者：
Wright, PE
影响因子：
56.9
作者：
Cluzel, P;Lebrun, A;Caron, F
通讯作者：
Caron, F