DNA profiling analysis of endometrial and ovarian cell lines reveals misidentification, redundancy and contamination.
DNA profiling analysis of endometrial and ovarian cell lines reveals misidentification, redundancy and contamination.
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DOI：
10.1016/j.ygyno.2012.06.017
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发表时间：
2012-10
影响因子：
4.7
通讯作者：
Bradford AP
中科院分区：
文献类型：
--
作者：
Korch C;Spillman MA;Jackson TA;Jacobsen BM;Murphy SK;Lessey BA;Jordan VC;Bradford AP
源自人类卵巢癌和子宫内膜癌的细胞系及其永生化非恶性细胞系是研究和表征妇科肿瘤发生分子机制并促进新型疗法开发的关键工具。为了确定错误识别、污染和冗余的程度，以及对基于这些模型的研究有效性的明显影响，我们对子宫内膜和卵巢细胞系进行了系统分析和编目。通过分析 DNA 微卫星短串联重复序列 (STR)、p53 核苷酸多态性和微卫星不稳定性来分析细胞系。对 51 个卵巢癌细胞系进行了分析，其中 10 个被发现是多余的，另外 5 个（A2008、OV2008、C13、SK-OV-4 和 SK-OV-6）被确定为宫颈癌细胞。对 10 种子宫内膜细胞系进行了分析，其中 RL-92、HEC-1A、HEC-1B、HEC-50、KLE 和 AN3CA 均表现出独特、未受污染的 STR 谱。通过 p53 基因突变测序，对 Ishikawa 和 ECC-1 子宫内膜癌细胞系的多种变体进行基因分型和分析。 ECC-1 细胞的特征与据报道源自的 EnCa-101 肿瘤不匹配，并且所有 ECC-1 分离株的基因型为 Ishikawa 细胞、MCF-7 乳腺癌细胞或其组合。两种正常的永生化子宫内膜上皮细胞系 HES 细胞和 hTERT-EEC 细胞系分别被鉴定为 HeLa 宫颈癌细胞和 MCF-7 乳腺癌细胞。结果表明，子宫内膜癌细胞系和卵巢癌细胞系存在明显的错误识别、重复和完整性丧失。通过 STR DNA 分析进行验证是一种简单且经济的方法，用于验证和验证使用这些模型进行的研究。
Cell lines derived from human ovarian and endometrial cancers, and their immortalized non-malignant counterparts, are critical tools to investigate and characterize molecular mechanisms underlying gynecologic tumorigenesis, and facilitate development of novel therapeutics. To determine the extent of misidentification, contamination and redundancy, with evident consequences for the validity of research based upon these models, we undertook a systematic analysis and cataloging of endometrial and ovarian cell lines. Profiling of cell lines by analysis of DNA microsatellite short tandem repeats (STR), p53 nucleotide polymorphisms and microsatellite instability. Fifty-one ovarian cancer lines were profiled with ten found to be redundant and five (A2008, OV2008, C13, SK-OV-4 and SK-OV-6) identified as cervical cancer cells. Ten endometrial cell lines were analyzed, with RL-92, HEC-1A, HEC-1B, HEC-50, KLE, and AN3CA all exhibiting unique, uncontaminated STR profiles. Multiple variants of Ishikawa and ECC-1 endometrial cancer cell lines were genotyped and analyzed by sequencing of mutations in the p53 gene. The profile of ECC-1 cells did not match the EnCa-101 tumor, from which it was reportedly derived, and all ECC-1 isolates genotyped as Ishikawa cells, MCF-7 breast cancer cells, or a combination thereof. Two normal, immortalized endometrial epithelial cell lines, HES cells and the hTERT-EEC line, were identified as HeLa cervical carcinoma and MCF-7 breast cancer cells, respectively. Results demonstrate significant misidentification, duplication, and loss of integrity of endometrial and ovarian cancer cell lines. Authentication by STR DNA profiling is a simple and economical method to verify and validate studies undertaken with these models.
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影响因子：
6.4
作者：
Carino, Cecilia;Olawaiye, Alexander B.;Cherfils, Salandre;Serikawa, Takehiro;Lynch, Maureen P.;Rueda, Bo R.;Gonzalez, Ruben R.
通讯作者：
Gonzalez, Ruben R.
影响因子：
3.8
作者：
Collins, Frances;MacPherson, Sheila;Saunders, Philippa T. K.
通讯作者：
Saunders, Philippa T. K.
DOI：
10.1016/0002-9378(92)91697-9
发表时间：
1992-02-01
影响因子：
9.8
作者：
BERCHUCK, A;RODRIGUEZ, G;BAST, RC
通讯作者：
BAST, RC
影响因子：
15.9
作者：
BAST, RC;FEENEY, M;KNAPP, RC
通讯作者：
KNAPP, RC
影响因子：
2.1
作者：
Barallon, Rita;Bauer, Steven R.;Butler, John;Capes-Davis, Amanda;Dirks, Wilhelm G.;Elmore, Eugene;Furtado, Manohar;Kline, Margaret C.;Kohara, Arihiro;Los, Georgyi V.;MacLeod, Roderick A. F.;Masters, John R. W.;Nardone, Mark;Nardone, Roland M.;Nims, Raymond W.;Price, Paul J.;Reid, Yvonne A.;Shewale, Jaiprakash;Sykes, Gregory;Steuer, Anton F.;Storts, Douglas R.;Thomson, Jim;Taraporewala, Zenobia;Alston-Roberts, Christine;Kerrigan, Liz
通讯作者：
Kerrigan, Liz