Recommendation of short tandem repeat profiling for authenticating human cell lines, stem cells, and tissues.
Recommendation of short tandem repeat profiling for authenticating human cell lines, stem cells, and tissues.
复制标题
DOI：
10.1007/s11626-010-9333-z
复制
发表时间：
2010-10
影响因子：
2.1
通讯作者：
Kerrigan, Liz
中科院分区：
文献类型：
--
作者：
Barallon, Rita;Bauer, Steven R.;Butler, John;Capes-Davis, Amanda;Dirks, Wilhelm G.;Elmore, Eugene;Furtado, Manohar;Kline, Margaret C.;Kohara, Arihiro;Los, Georgyi V.;MacLeod, Roderick A. F.;Masters, John R. W.;Nardone, Mark;Nardone, Roland M.;Nims, Raymond W.;Price, Paul J.;Reid, Yvonne A.;Shewale, Jaiprakash;Sykes, Gregory;Steuer, Anton F.;Storts, Douglas R.;Thomson, Jim;Taraporewala, Zenobia;Alston-Roberts, Christine;Kerrigan, Liz
自从细胞被用作研究工具以来，细胞的错误识别和交叉污染一直困扰着生物医学研究。错误鉴定的细胞系的例子继续浮出水面，直到今天。通过提高对这一问题的认识和要求科学家自愿采取适当行动来根除这一问题的努力尚未取得成功。非生物多样性细胞认证是科学过程中的一个重要步骤，在对提交出版的论文进行同行评审或对提交供资的赠款进行审查期间，应该是一个固有的考虑因素。为了促进适当的身份测试，必须提供准确、可靠、廉价和标准化的细胞和细胞系鉴定方法。为此，一个国际科学家团队正在准备一个关于使用短串联重复序列（STR）分析鉴定人类细胞的共识标准。该标准将作为美国国家标准提交美国国家标准协会审查和批准，将为研究人员提供使用STR分析鉴定人类细胞系的指导。这种指导将包括DNA样本制备方法的细节、待评价基因座的适当数量和类型以及结果的解释和质量控制。与标准本身相联系的是在国家生物技术信息中心的主持下建立和维持一个公开的STR概况数据库。预计该共识标准将被授权机构和科学期刊采用，作为鉴定人类细胞系、干细胞和组织的适当方法。
Cell misidentification and cross-contamination have plagued biomedical research for as long as cells have been employed as research tools. Examples of misidentified cell lines continue to surface to this day. Efforts to eradicate the problem by raising awareness of the issue and by asking scientists voluntarily to take appropriate actions have not been successful. Unambiguous cell authentication is an essential step in the scientific process and should be an inherent consideration during peer review of papers submitted for publication or during review of grants submitted for funding. In order to facilitate proper identity testing, accurate, reliable, inexpensive, and standardized methods for authentication of cells and cell lines must be made available. To this end, an international team of scientists is, at this time, preparing a consensus standard on the authentication of human cells using short tandem repeat (STR) profiling. This standard, which will be submitted for review and approval as an American National Standard by the American National Standards Institute, will provide investigators guidance on the use of STR profiling for authenticating human cell lines. Such guidance will include methodological detail on the preparation of the DNA sample, the appropriate numbers and types of loci to be evaluated, and the interpretation and quality control of the results. Associated with the standard itself will be the establishment and maintenance of a public STR profile database under the auspices of the National Center for Biotechnology Information. The consensus standard is anticipated to be adopted by granting agencies and scientific journals as appropriate methodology for authenticating human cell lines, stem cells, and tissues.
登录
查看更多内容
影响因子：
6.1
作者：
Nardone, Roland M.
通讯作者：
Nardone, Roland M.
影响因子：
6.4
作者：
Capes-Davis, Amanda;Theodosopoulos, George;Freshney, R. Ian
通讯作者：
Freshney, R. Ian
影响因子：
5.8
作者：
Schweppe, Rebecca E.;Klopper, Joshua P.;Haugen, Bryan R.
通讯作者：
Haugen, Bryan R.
DOI：
10.1073/pnas.121616198
发表时间：
2001-07-03
影响因子：
11.1
作者：
Masters, JR;Thomson, JA;Debenham, PG
通讯作者：
Debenham, PG
影响因子：
56.9
作者：
NELSONRE.WA;FLANDERM.RR;HAWTHORN.PK
通讯作者：
HAWTHORN.PK