Temporal analysis of vascular smooth muscle cell elasticity and adhesion reveals oscillation waveforms that differ with aging.
Temporal analysis of vascular smooth muscle cell elasticity and adhesion reveals oscillation waveforms that differ with aging.
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DOI：
10.1111/j.1474-9726.2012.00840.x
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发表时间：
2012-10
期刊：
影响因子：
7.8
通讯作者：
Meininger GA
中科院分区：
文献类型：
--
作者：
Zhu Y;Qiu H;Trzeciakowski JP;Sun Z;Li Z;Hong Z;Hill MA;Hunter WC;Vatner DE;Vatner SF;Meininger GA
A spectral analysis approach was developed for detailed study of time-resolved, dynamic changes in vascular smooth muscle cell (VSMC) elasticity and adhesion to identify differences in VSMC from young and aged monkeys. Atomic force microscopy (AFM) was used to measure Young's modulus of elasticity and adhesion as assessed by fibronectin (FN) or anti-beta 1 integrin interaction with the VSMC surface. Measurements demonstrated that VSMC cells from old versus young monkeys had elevated elasticity (21.6 kPa vs 3.5 kPa or a 612% elevation in elastic modulus) and adhesion (86 pN vs 43 pN or a 200% increase in unbinding force). Spectral analysis identified three major frequency components in the temporal oscillation patterns for elasticity (ranging from 1.7×10-3 to 1.9×10-2 Hz in old and 8.4×10-4 to 1.5×10-2 in young) and showed that the amplitude of oscillation was larger (p<0.05) in old than in young at all frequencies. It was also observed that patterns of oscillation in the adhesion data were similar to the elasticity waveforms. Cell stiffness was reduced and the oscillations were inhibited by treatment with cytochalasin D, ML7 or blebbistatin indicating involvement of actin-myosin driven processes. In conclusion, these data demonstrate the efficacy of time-resolved analysis of AFM cell elasticity and adhesion measurements and that it provides a uniquely sensitive method to detect real-time functional differences in biomechanical and adhesive properties of cells. The oscillatory behavior suggests mechanisms governing elasticity and adhesion are coupled and affected differentially during aging which may link these events to changes in vascular stiffness.
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DOI：
10.1038/nrm2957
发表时间：
2010-09
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
通讯作者：
--
影响因子：
3.5
作者：
Crick, S. L.;Yin, F. C. -P.
通讯作者：
Yin, F. C. -P.
DOI：
10.1152/ajplung.00043.2003
发表时间：
2003-12-01
影响因子：
4.9
作者：
Kuo, KH;Dai, JZ;van Breemen, C
通讯作者：
van Breemen, C
影响因子：
37.8
作者：
Qiu, Hongyu;Depre, Christophe;Vatner, Stephen F.
通讯作者：
Vatner, Stephen F.
DOI：
10.1083/jcb.200204153
发表时间：
2002-11-25
期刊：
The Journal of cell biology
影响因子：
--
作者：
Galbraith CG;Yamada KM;Sheetz MP
通讯作者：
Sheetz MP